人脐血间充质干细胞在脑瘫大鼠脑内定植和存活能力的研究  

Study of the colonizing and survivability of human umbilical cord blood mesenchymal stem cells in brain of rats with cerebral palsy

作　　者：杨自金[1] 卢思广[1] 高长龙[1] 郭佳丽[1] 苗莉[1] 李红梅[1] YANG Zi-jin LU Si-guang GAO Chang-long GUO Jia-li MIAO Li LI Hong-mei(Department of Pediatrics, The First Hospital of Lianyungang ,Lianyungang Jiangsu 222000, Chin)

机构地区：[1]江苏省连云港市第一人民医院儿内科,222000

出　　处：《蚌埠医学院学报》2016年第8期985-987,991,共4页Journal of Bengbu Medical College

基　　金：江苏省连云港市科技局重点科室建设(SH1117)

摘　　要：目的：通过侧脑室注射,观察人脐血间充质干细胞（MRCs）在脑瘫（CP）大鼠脑内的存活、迁移、分化情况。方法：用腹腔注射脂多糖方法制作CP大鼠模型14只,将标记过的MSCs经侧脑室注射到12只CP大鼠脑内,观察在注射1-4周后MSCs在脑内存活、迁移、分化情况;2只不进行注射的CP大鼠作空白对照。结果：腹腔注射脂多糖方法成功制作CP模型;MSCs鉴定成功;MSCs经侧脑室注射能在CP大鼠脑内存活、迁移,并分化成神经细胞,随移植时间推移,有减少趋势（P〈0.01）。2只未注射模型大鼠未检测到MSCs阳性细胞。结论：MSCs能在CP大鼠脑内定植,随着时间推移,数量明显减少。Objective： To explore the viability,migration and differentiation of human umbilical cord blood mesenchymal stem cells（ MSCs） in brain of rats with cerebral palsy（ CP） after the lateral ventricle injection. Methods： Fourteen CP model rats were established by the intraperitoneal injection of lipopolysaccharide,and the labeled MSCs were injected into the lateral ventricle in 12 CP rats. The viability,migration and differentiation of MSCs in rat brain were observed after 1 to 4 weeks of injection. Other two rats without injecting were set as the control group. Results： The CP model rats were successfully established by the intraperitoneal injection of lipopolysaccharide,and the MSCs were identified. The colonizing MSCs could survive,migrate and differentiate into neurons,and which decreased with the transplanting time extending（ P〈0. 01）. The positive MSCs was not explored in two rats without the MSCs injecting.Conclusions： MSCs can survive in brain of rats with CP,and the number of which decrease with the time extending.

关 键 词：脑瘫 脐血间充质干细胞 定植 大鼠 

分 类 号：R742[医药卫生—神经病学与精神病学]