三倍体葡萄胚挽救苗驯化及移栽技术研究  

Study on Domesticating and Transplanting Techniques of Triploid Grape Seedlings from Embryo Rescue

作　　者：路凤珍 温晓敏[1,2] 张娜[2] 陈三春 黄建全[2] 田淑芬[2] 

机构地区：[1]天津师范大学生命科学学院,天津300387 [2]天津市农业科学院葡萄研究中心天津市葡萄遗传与育种企业重点实验室,天津300192

出　　处：《天津农业科学》2016年第10期92-95,共4页Tianjin Agricultural Sciences

基　　金：天津市农业科学院院长基金(15010)

摘　　要：以玫瑰香优系×早甜、玫瑰香优系×户太8号、玫瑰香优系×醉金香、玫瑰香优系×巨玫瑰杂交的胚挽救苗为试材,系统地研究了基质种类和组成、生根粉浓度、营养液浓度、胚挽救苗苗龄、不同杂交组合5个方面对三倍体葡萄胚挽救苗移栽成活率的影响。结果表明,珍珠岩∶蛭石∶河沙=1∶1∶1作为基质的移栽成活率显著高于其他处理组;300 mg·L-1生根(ABT)溶液浸泡根系30s的移栽成活率最高;1/8MS营养液处理胚挽救苗的成活率最高,为71.67%;培养40 d的胚挽救苗移栽成活率为65.00%,显著高于其他试验组;玫瑰香优系×醉金香杂交苗移栽成活率最高。初步探索出一套简单易行、实用高效的葡萄胚挽救苗优化配套移栽技术。Grape seedlings from hybrids of Muscat Hamburg x Zaotian, Muscat Hamburg x Hutai No.8, Muscat Hamburg x Zuijinxi- ang, Muscat Hamburg x Jumeigui seedless grapes through embryo rescue were taken as experimental materials. The effects of trans- plant matrix, rooting powder concentration, the concentration of nutrient solution, seedling transplant stages and different cross combi- nation on transplant survival percent of triploid grape embryo rescue seedlings were systematically studied. The results showed that the transplanting survival rate with perlite ：vermiculite ：sand =1 ：1：1 matrix was significantly higher than other treatment groups; the sur- vival rate were best with 300 mg· L-1 ABT solution soaking for 30 s; the survival rate of embryo rescue seedlings treated with 1/SMS nutrient solution was the highest, which was 71.67%;survival rate of embryo rescue seedlings of 40 d was 65%, which was significant- ly higher than other experimental groups; the transplanting survival rate of the hybrid seedlings of Muscat Hamburg x Znijinxiang was the highest. A set of simple, available, practical and efficient technique of embryo rescue seedlings was obtained.

关 键 词：三倍体葡萄 胚挽救苗 移栽 

分 类 号：S663.1[农业科学—果树学]