灵芝单、双核菌丝差异研究  

Study on the differences of monokaryotic mycelium and dikaryotic mycelium of Ganoderma lucidum

作　　者：叶丽云[1] 鲁欣[1] 林强[1] 吴小平[1] 

机构地区：[1]福建农林大学菌物研究中心,福建福州350002

出　　处：《食品工业科技》2016年第20期211-215,共5页Science and Technology of Food Industry

基　　金：福建省种业创新与产业化工程专项项目(2014S1477-21)

摘　　要：通过菌丝形态特征、生长速度以及多糖和三萜含量的差异分析灵芝单核、双核的差异,同时应用高效液相色谱法比较了灵芝单核、双核菌丝指纹图谱,并采用实时荧光定量PCR技术研究三萜关键酶基因表达量。结果显示,双核菌株J-7/AL-2菌丝生长旺盛且整齐,单核菌株菌丝长势较弱。双核菌株的生长速度(0.55 cm/d)明显快于两株单核菌株(p<0.05)。单核菌株J-7多糖含量(42.11 mg/g)较双核菌株高出20.8%。双核菌株三萜含量(42.54 mg/g)显著高于单核菌株30%左右(p<0.05)。高效液相色谱结果显示,双核菌株与两株单核菌株在三萜组分上存在一定的差异,相似度为0.82;两株单核菌株的菌丝三萜组分相似度极高,相似度达0.96。实时荧光定量PCR结果显示,双核菌株的6个三萜关建酶基因的表达量都要显著高于其他两株单核菌株,说明基因表达可能存在协调增效作用。The morphological characteristics, growth rate, and the difference of the contents of polysaccharides and triterpenoids of monokaryotic mycelium and dikaryotic mycelium of Ganoderma lucidum were compared. Meanwhile,the fingerprint of triterpenoids of Ganoderma lucidum was analyzed by high performance liquid chromatography（HPLC） method and the expression levels of six triterpeniods enzyme genes were analyzed by real-time quantitative PCR.The result showed that the dikaryotic strain J-7/AL-2 grew vigorously and neatly while monokaryotic strain grew weaker.The growth rate of dikaryotic strain （0.55 cm/d）was significantly faster than that of the two strains （ p 〈 0.05）.The content of polysaccharide of monokaryotic strain J-7 （42.54mg/g） was higher than d ikaryotic strain out of 20.8%.The content of the triterpenoids （42.54 mg/g）was significantly higher than that of monokaryotic strain about 30% （p 〈 0.05）.The result of HPLC showed that there was a certain difference between the fingerprints of dikaryotic strain and two monokaryotic strains and the similarity was 0.82. But the triterpenoids compounds of two monokaryotic strains had the highest similarity at 0.96.The result of real-time quantitative PCR showed that the expression of six triterpeniods enzyme genes in dikaryotic strain J-7/AL-2 was much more than two monokaryotic strains because of synergistic gene expression effect.

关 键 词：单核菌丝 双核菌丝 多糖 三萜 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]