棘豆素对人雄激素非依赖性前列腺癌PC-3细胞的作用研究  

2',4'-dihydroxychalcone-induced apoptosis of PC-3 human prostate cancer cells via up-regulation of P27kip1 and PTEN

作　　者：沈丰[1] 王艳[1] 邹明畅[1] 盛玉青[1] 

机构地区：[1]镇江市第一人民医院药剂科,江苏镇江212002

出　　处：《安徽医药》2016年第8期1462-1465,共4页Anhui Medical and Pharmaceutical Journal

摘　　要：目的研究棘豆素(2',4'-dihydroxychalcone,TFC)对人雄激素非依赖性前列腺癌PC-3细胞的作用,并探讨其作用机制。方法 CCK-8法检测药物对细胞增殖的影响;Hoechst 33258染色、荧光显微镜观察细胞的形态学变化;流式细胞术分析细胞凋亡率和细胞周期的变化;Western blot检测药物对肿瘤细胞中P27kip1和PTEN蛋白表达的影响。结果 TFC能显著抑制PC-3细胞的增殖,细胞呈现凋亡形态学改变,凋亡率明显增加,细胞周期阻滞于G0/G1期。此外,P27kip1和PTEN蛋白表达量明显增加。结论 TFC通过诱导PC-3细胞凋亡抑制肿瘤细胞增殖,可能与其G0/G1细胞周期阻滞,以及PTEN和P27Kip1蛋白表达上调有关。Objective To explore the effect of 2′,4′-dihydroxychalcone （TFC）on androgen-independent prostate cancer PC-3 cells and its possible anti-cancer mechanism.Methods The CCK-8 assay was employed for cellular proliferation measurement,Hoechst 33258 dye and fluorescent microscope for morphological observation,flow cytometry （FCM）for detection of apoptosis and cell cycles, Western blot for analyzing the expression of P27kip1 and PTEN proteins in PC-3 cells treated with TFC of different concentration.Re-sults TFC significantly inhibited the proliferation of PC-3 cells,induced apoptotic morphological features,increased the percentage of apoptosis,arrested cell cycle in G0 /G1 phase,up-regulated the expression of P27kip1 and PTEN proteins in a concentration dependent manner.Conclusions TFC could inhibit the proliferation and induce the apoptosis in PC-3 cells by up-regulating P27kip1 and PTEN protein and arresting cell cycle in G0 /G1 phase.

关 键 词：前列腺肿瘤 黄酮类 棘豆属 周期素依赖激酶抑制剂P27 基因 肿瘤抑制 细胞凋亡 

分 类 号：R737.25[医药卫生—肿瘤]