细胞信号转导抑制因子1沉默增强人口腔癌细胞对化疗药物的敏感性  

Effects of suppressor of cytokine signaling-1 silencing on the chemotherapeutic drugs sensitivity of human oral cancer

作　　者：夏凤君[1] 申铁兵[1] 

机构地区：[1]内蒙古医科大学附属医院口腔科,内蒙古呼和浩特010050

出　　处：《安徽医药》2016年第8期1544-1547,共4页Anhui Medical and Pharmaceutical Journal

摘　　要：目的研究细胞因子信号转导抑制因子1(SOCS1)基因沉默对人口腔癌细胞化疗药物敏感性的影响及相关机制。方法Western blotting及实时荧光定量(PCR)验证SOCS1干扰序列沉默人口腔表皮样癌细胞(KB细胞)株中SOCS1的表达水平;MTT比色法分析细胞对化疗药物敏感性的变化;流式细胞术检测细胞凋亡。结果将SOCS1干扰片段转染KB细胞后,SOCS1 mRNA及蛋白表达水平均明显降低。沉默SOCS1表达后,化疗药物5-氟尿嘧啶和长春新碱对KB细胞的半数抑制浓度(IC50)均显著变小(P<0.05),SOCS1沉默还可增加长春新碱诱导的细胞凋亡(P<0.05),SOCS1表达抑制后KB细胞核转录因子-κB(NF-κB)和半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)的蛋白表达水平明显降低,与对照组比较差异均有统计学意义(P<0.05)。结论SOCS1表达有效抑制后,人口腔癌细胞KB对化疗药物的敏感性增强,且促进化疗药物诱导的肿瘤细胞凋亡。Objective To investigate the changes of chemotherapeutic drugs’sensitivity of human oral cancer after suppressor of cyto-kine signaling-1 （SOCS1）gene silencing.Methods Western blotting and real-time PCR were used to verify the downregulation ofSOCS1 human oral cancer cell line （KB）after transfection;and the change of chemotherapeutic drugs’sensitivity was detected by MTT assay;flow cytometry was used to detect the cell apoptosis rate.Results of RT-PCR and Western blotting showed that the expression levels of SOCS1 mRNA and protein were significantly decreased in small interfering SOCS1 （SOCS1-siRNA）transfected KB cells.After the silence of SOCS1,the median inhibitory concentration of chemotherapeutic drugs （5-fluorouracil and vincristine）for KB cells de-creased significantly （P 〈0.05）.The cell apoptosis rate of vincristine for KB cells significantly increased after SOCS1 gene silencing （P 〈0.05）.The NF-κB and Caspase-3 protein expression levels in the experiment group dropped significantly compared with that of control group （P 〈0.05）.Conclusions Inhibition of SOCS1 gene expression of oral cancer KB cells enhances the sensitivity to che-motherapeutic drugs,and promotes the apoptosis of cancer cells induced by chemotherapeutic drugs.

关 键 词：口腔肿瘤 细胞因子信号转导蛋白抑制因子 基因沉默 氟尿嘧啶 长春新碱 

分 类 号：R739.8[医药卫生—肿瘤]