嗜吡啶红球菌GF3降解1-氨基蒽醌-2-磺酸的特性  

Biodegradation of 1-amino-anthraquinone-2-sulfonic acid by Rhodococcus pyridinivorans GF3

作　　者：臧苗苗[1] 吕红[1] 周集体[1] 管玉江[2] 王竞[1] 关小凡[1] 郭婉茜[3] 

机构地区：[1]大连理工大学环境学院工业生态与环境工程教育部重点实验室,大连116024 [2]台州学院生命科学学院,台州317000 [3]哈尔滨工业大学城市水资源与水环境国家重点实验室,哈尔滨150090

出　　处：《环境科学学报》2016年第10期3643-3650,共8页Acta Scientiae Circumstantiae

基　　金：哈尔滨工业大学城市水资源与水环境国家重点实验室开放基金项目(No.QA201412)~~

摘　　要：从受溴氨酸污染的泥土中分离出1株蒽醌染料中间体1-氨基蒽醌-2-磺酸(简称ASA-2)降解菌株GF3.经形态学观察和16S r DNA序列分析,鉴定该菌株为嗜吡啶红球菌.研究了该菌对ASA-2脱色的特性,并利用液相-质谱联用仪初步分析了ASA-2降解终产物.研究结果表明,外加蛋白胨、酵母膏和水解酪蛋白均能促进ASA-2的生物脱色,其中蛋白胨促进作用最为明显.进一步的研究发现多种氨基酸可加速ASA-2的生物脱色过程,其中L-亮氨酸促进效果最好.ASA-2脱色的最适环境条件为p H 8.0、30℃和150 r·min^(-1).最适条件下,菌株GF3可使108 mg·L-1的ASA-2在30 h内脱色率达95%以上,TOC去除率为62%.紫外-可见波谱显示,当ASA-2水溶液由红色褪成无色时,ASA-2的特征吸收峰完全消失,并在340 nm产生了新峰.进一步分析发现,ASA-2降解终产物质荷比为260,初步推测产物为3-氨基-4-磺酸基邻苯二甲酸.此外,菌株GF3还可以降解溴氨酸、蒽醌-2-磺酸钠和蒽醌-2-羧酸.An anthraquinone dye intermediate 1-amino-anthraquinone-2-sulfonic acid（ ASA-2）-degrading strain GF3 was isolated from the bromamine acid-contaminated soil samples. Based on morphological observation and 16 S r DNA sequence analysis,strain GF3 was identified as Rhodococcus pyridinivorans. The decolorization of ASA-2 by strain GF3 was studied and ASA-2 degradation product was analyzed using high performance liquid chromatography-mass spectrometry（ HPLC-MS）. The results show that additional peptone,yeast extract and casamino acid could promote ASA-2 biodecolorization,among which the accelerating effect of peptone was the most obvious. Further experimental results demonstrate that many amino acids could accelerate the process of ASA-2 bio-decolorization,and L-leucine had the most significantly accelerating effect on ASA-2 decolorization and cell growth.The optimal environmental conditions for ASA-2 bio-decolorization were p H 8.0,30 ℃ and 150 r·min^（-1）. Under the optimal conditions,the decolorization efficiency of 108 mg·L-1ASA-2 could reach over 95% in 30 h,and TOC removal rate was 62%. In UV-Visible spectra,the characteristic absorption peaks of ASA-2 totally disappeared and a new peak at 340 nm appeared when the red color of ASA-2 aqueous solution faded to be colorless. Further analysis found that the mass to charge ratio of the end product from ASA-2 degradation was 260. Thus,the degradation product was proposed to be 3-amino-4-sulfophthalic acid. In addition,strain GF3 could also degrade bromamine acid,anthraquinone-2-sulfonate and anthraquinone-2-carboxylic acid.

关 键 词：1-氨基蒽醌-2-磺酸 嗜吡啶红球菌GF3 降解 

分 类 号：X172[环境科学与工程—环境科学]