PTEN基因在猪卵母细胞、早期胚胎和各主要脏器的表达情况  

Expression of PTENgene in porcine oocytes,pre-implantation embryos and main organs

作　　者：王华[1] 曹筠青 唐博[1] 张学明[1] 李子义[1,2] 

机构地区：[1]吉林大学动物医学学院,吉林长春130062 [2]吉林大学第一医院转化医学研究院/人类疾病动物模型国家地方联合工程实验室,吉林长春130061

出　　处：《中国兽医学报》2016年第10期1774-1778,共5页Chinese Journal of Veterinary Science

基　　金：国家重点基础研究发展计划资助项目(973计划)(2011CB944204)

摘　　要：PTEN基因在哺乳动物早期胚胎发育中起重要作用,但PTEN基因在猪的卵母细胞、早期胚胎和各主要脏器的表达情况还未见报道。本研究以猪为研究对象,利用非同位素银染DNA测序和荧光定量PCR等技术,对PTEN基因在猪卵母细胞、猪体外受精胚胎和仔猪各主要脏器的表达水平进行了研究。结果表明:PTEN基因在猪GV期卵母细胞、MII期卵母细胞和早期胚胎发育中持续表达,在桑椹胚期高表达,说明PTEN基因在胚胎发育过程中尤其在胚胎致密化时期发挥重要作用;PTEN基因在仔猪各主要脏器均有表达,在肺脏高表达,说明PTEN基因对仔猪主要脏器发育尤其是对仔猪肺脏的发育发挥重要作用。试验结果为今后PTEN基因在猪卵母细胞、早期胚胎和仔猪各主要脏器的研究奠定了基础。The PTEN gene plays an important role in early embryo development in mammals. However,the expression of PTEN gene in pig oocytes, early embryos and main organs has not been reported. To study the differences of the expression levels of PTEN gene in porcine oocytes, in vitro fertilized embryos and piglet main organs,we used pig as research subject,the PTEN gene expressions in porcine oocytes, in vitro fertilized embryos and piglet main organs were studied through silver sequence DNA technology and real time RCR. The results showed that the PTEN mRNA persistently expressed in geminal vesicle （GV） oocytes, metapbase II （MII） oocytes, and early embryos,and PTEN mRNA level peaked in morular stage,indicating that PTEN gene plays an important role in the process of embryonic developments,especially in in embryonic compaclion period. PTEN mRNA was detected in all main organs, but highly expressed in lungs, suggesting that PTEN gene may be involved in piglet main organs development, especially in lung develop- ment. The results laid a foundation for subsequent PTEN research in pig oocytes, early embryos and main organs.

关 键 词：猪 PTEN基因 卵母细胞 体外受精胚胎 主要脏器 

分 类 号：S852[农业科学—基础兽医学]