脑胶质瘤患者外周血中Th17、Th22细胞的表达及意义  

Expressions and significance of Th17 and Th22 cells in peripheral blood of patients with glioma

作　　者：满琦[1] 张安[1] 张源[2] 樊明德[1] 马道新[3] 王成伟[1] 

机构地区：[1]山东大学第二医院神经外科,山东济南250033 [2]山东大学齐鲁医院急诊科,山东济南250012 [3]山东大学齐鲁医院血液科,山东济南250012

出　　处：《山东大学学报（医学版）》2016年第10期55-59,70,共6页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金(ZR2013HM083)

摘　　要：目的探究脑胶质瘤患者外周血中Th17、Th22细胞的表达及意义。方法取34例脑胶质瘤患者及24例健康对照者为研究对象,用流式细胞术检测外周血中Th17、Th22细胞的表达比例。用实时荧光定量逆转录聚合酶链反应(RT-PCR)技术检测外周血单个核细胞中视黄酸相关孤儿核受体(RORC)mRNA及芳香烃受体(AHR)mRNA的表达。用酶联免疫吸附分析(ELISA)检测血浆中Th22细胞相关因子白细胞介素-22(IL-22)的水平。结果与健康对照组相比,脑胶质瘤患者外周血Th17细胞比例(占淋巴细胞)升高不明显(P>0.05),且高级别组(WHOⅢ-Ⅳ级)与低级别组(WHOⅠ-Ⅱ级)之间差异不明显(P>0.05);Th22细胞比例(占淋巴细胞)明显升高(P<0.05),且不同级别之间差异明显(P<0.05)。脑胶质瘤患者Th17与Th22细胞呈正相关(r=0.40,P=0.03),健康对照组中二者无相关(P>0.05)。与健康对照组比较,患者外周血中RORC mRNA及AHR mRNA的升高不明显(P>0.05,P>0.05),且不同级别间差异不明显(P>0.05,P>0.05)。患者血浆IL-22水平比健康对照组明显升高(P<0.05),但不同级别之间差异不明显(P>0.05)。结论脑胶质瘤患者外周血中存在Th22细胞及IL-22异常升高,Th17细胞与Th22细胞可能共同参与胶质瘤的发生发展过程。Objective To investigate the expression and significance of Thl7 and Th22 cells in the peripheral blood of patients with glioma. Methods A total of 34 patients with glioma and 24 normal controls were enrolled. The percenta- ges of Thl7 and Th22 cells in peripheral blood were measured by flow cytometry. Retinoid-related orphan nuclear receptor （RORC） and arylhydrocarbon receptor （AHR） mRNA in peripheral blood mononuclear cells were detected by reverse-transcriptional polymerase chain reaction （RT-PCR）. The plasma level of interleukin-22 （IL-22） was detected by ELISA. Results There was no difference between patients and healthy controls in circulating Thl7 cells （P 〉 0.05 ）, and no difference was found between the high-grade group （ WHO Ⅲ -Ⅳ ） and low-grade group （ WHO Ⅰ - Ⅱ ） （ P 〉 0.05 ）. The percentage of circulating Th22 cells in glioma was significantly increased compared with that in controls （ P 〈 0.05 ）, and the difference between different grade groups was obvious （ P 〈 0.05 ）. A positive correlation between Th22 and Thl7 cells was observed in glioma （ r = 0. 40, P = 0.03 ）, no correlation was found in controls （ P 〉 0.05 ）. The difference of RORC and AHR between patients and controls was not significant （ P 〉 0. 05, P 〉 0.05 ）,and no difference was found between different grade groups （ P 〉 0. 05, P 〉 0.05 ）. The serum IL-22 level in patients was significantly increased compared with that in controls （ P 〈 0.05 ）. However, there was no difference of serum IL-22 levels between different grade groups （P 〉 0.05）. Conclusion There exist abnormal increases of Th22 cells and IL-22 levels in the peripheral blood of glioma, and Thl7 and Th22 cells may jointly participate in the pathogenesis and progression of glioma.

关 键 词：脑胶质细胞瘤 TH17细胞 TH22细胞 白细胞介素-22 

分 类 号：R739.41[医药卫生—肿瘤]