拟南芥NUP107-160亚复合物突变体表型分析  

The Phenotype Analysis of NUP107-160 Subcomplex Mutants in Arabidopsis

作　　者：肖龙[1] 刘文瑄 陈福禄[3] 张晓玫[3] 陈庆山[1] 傅永福[3] 

机构地区：[1]东北农业大学农学院,哈尔滨150030 [2]北京林业大学生物科学与技术学院,北京100083 [3]中国农业科学院作物科学研究所,农业部北京大豆生物学重点实验室,北京100081

出　　处：《中国农业科技导报》2016年第5期54-61,共8页Journal of Agricultural Science and Technology

基　　金：国家自然科学基金项目(31370324,30670189和31571411)资助

摘　　要：核孔复合物(NPC)是真核细胞核膜上比较大的蛋白复合物之一,它通过调节核质间的物质运输,参与生物的多种生长发育过程。目前,植物中只有少数NPC组分的功能得到了初步分析。通过对NUP107-160亚复合物中各组分突变体的鉴定,发现nup160、nup96、nup85、nup107和seh1突变体的叶片振动周期延长,主根的伸长受到抑制,其中nup160、nup96和nup107三种突变体在长日照条件下开花时间显著提前。结果表明NUP107-160亚复合物可能参与拟南芥生物节律、根生长和开花时间的调节。Nuclear pore complex（ NPC） is one of the large protein complex in nuclear envelope,and is involved in lots of growth and developmental processes through gating nucleocytoplasmic trafficking of RNA and proteins in various organisms. Currently,only several components function of NPC have been analyzed in plants. Though identifying the mutants of all members in NUP107-160 subcomplex and analyzing their phenotypes,this paper found that mutants of nup160,nup96,nup85,nup107,and seh1 had longer circadian periods of leaf movement and shorter primary roots. But,only three mutants,nup160,nup96,and nup107,significantly displayed early flowering. The results indicated that NUP107- 160 subcomplex might be a novel player for the regulation of circadian clock,root elongation,and flowering time in Arabidopsis.

关 键 词：核孔复合物 NUP107-160亚复合物 核孔蛋白 生物钟 根 开花 拟南芥 

分 类 号：Q816[生物学—生物工程]