利用SSR荧光标记分析山西糯玉米农家种的遗传多样性  

Analysis of Genetic Diversity of Waxy Corn Landraces in Shanxi Using Fluorescent SSR Markers

作　　者：李锐[1] 白建荣[1,2,3] 张丛卓[1] 张效梅 闫蕾[1] 杨瑞娟[1] LI Rui BAI Jianrong ZHANG Congzhuo ZHANG Xiaomei YAN Lei YANG Ruijuan(Institute of Crop Sciences, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China Research Center of Modern Agriculture, Shanxi Academy of Agricuhural Sciences, Taiyuan 030031, China Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan 030031, China)

机构地区：[1]山西省农业科学院作物科学研究所,山西太原030031 [2]山西省农业科学院现代农业研究中心,山西太原030031 [3]农业部黄土高原作物基因资源与种质创新重点实验室,山西太原030031

出　　处：《山西农业科学》2016年第10期1433-1436,共4页Journal of Shanxi Agricultural Sciences

基　　金：山西省科技攻关项目(20130311002-8)

摘　　要：采取混合取样策略,利用荧光SSR技术对来源于山西省的45份糯玉米地方品种和40个位点进行了分析。结果表明,在45个糯玉米地方品种中,共检测出等位变异241个,每个位点检测出3~11个等位变异,平均每个位点6个;多态性信息量（PIC）变化范围在0.27~0.87,平均每个位点0.71;标记索引指数（MI）变化范围在1.35~9.53,平均每个位点4.42;聚类分析可将45份糯玉米地方品种划分为4个类群。结果显示,部分品种有许多特有的等位变异,应引起特别的注意,需作进一步的分析与研究。Genetic diversity and genetic variation of waxy corn landraces in Shanxi were analyzed with 40 fluorescent SSR loci by using a bulk sampling strategy. The result showed that totally 241 alleles in 45 corn landraces were detected with an average of 6 ranged from 3 to 11 per locus. PIC among them ranged from 0.27 to 0.87 with an average of 0.71 per locus. MI among them ranged from 1.35 to9.53 with an average of 4.42 per locus. The 45 landraces were clustered into 4 groups. The results showed that some of them had their particular alleles, so that they should be paid more to study further.

关 键 词：SSR荧光标记 糯玉米 地方品种 遗传多样性 山西 

分 类 号：S513.032[农业科学—作物学]