One-year water-ageing of calcium phosphate composite containing nano-silver and quaternary ammonium to inhibit biofilms  

作　　者：Lei Cheng Ke Zhang Chen-Chen Zhou Michael D Weir Xue-Dong Zhou Hockin HK Xu 

机构地区：[1]State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China [2]Biomaterials and Tissue Engineering Division, Department of Endodontics, Periodontics and Prosthodontics, University of Maryland School of Dentistry, Baltimore, USA [3]Department of Orthodontics, School of Stomatology, Capital Medical University, Beijing, China [4]Center for Stem Cell Biology and Regenerative Medicine, University of Maryland School of Medicine, Baltimore, USA [5]Department of Mechanical Engineering, University of Maryland, Baltimore County, USA

出　　处：《International Journal of Oral Science》2016年第3期172-181,共10页国际口腔科学杂志（英文版）

基　　金：supported by the International Science and Technology Cooperation Program of China (2014DFE30180) (Xue-Dong Zhou);National Natural Science Foundation of China grant 81430011 (Xue-Dong Zhou),81372889 (Lei Cheng),81400540 (Ke Zhang);?nancial support from the School of Stomatology at the Capital Medical University in China (Ke Zhang),NIH R01 DE17974 (Hockin HK Xu);a seed grant from the University of Maryland School of Dentistry (Hockin HK Xu)

摘　　要：Dental composites are commonly used restorative materials; however, secondary caries due to biofilm acids remains a major problem. The objectives of this study were （1） to develop a composite containing quaternary ammonium dimethacrylate （QADM）, nanoparticles of silver （NAg）, and nanoparticles of amorphous calcium phosphate （NACP）, and （2） to conduct the first investigation of the mechanical properties, biofilm response and acid production vs water-ageing time from 1 day to 12 months. A 4 x 5 design was utilized, with four composites （NACP-QADM composite, NACP-NAg composite, NACP-QADM-NAg composite, and a commercial control composite）, and five water-ageing time periods （1 day, and 3, 6, 9, and 12 months）. After each water- ageing period, the mechanical properties of the resins were measured in a three-point flexure, and antibacterial properties were tested via a dental plaque biofilm model using human saliva as an inoculum. After 12 months of water-ageing, NACP-QADM- NAg had a flexural strength and elastic modulus matching those of the commercial control （P〉 0.1）. Incorporation of QADM or NAg into the NACP composite greatly reduced biofilm viability, metabolic activity and acid production. A composite containing both QADM and NAg possessed a stronger antibacterial capability than one with QADM or NAg alone （P〈0.05）. The anti-biofilm activity was maintained after 12 months of water-ageing and showed no significant decrease with increasing time （P〉0.1）. In conclusion, the NACP-QADM-NAg composite decreased biofilm viability and lactic acid production, while matching the load- bearing capability of a commercial composite. There was no decrease in its antibacterial properties after 1 year of water-ageing. The durable antibacterial and mechanical properties indicate that NACP-QADM-NAg composites may be useful in dental restorations to combat caries.Dental composites are commonly used restorative materials; however, secondary caries due to biofilm acids remains a major problem. The objectives of this study were （1） to develop a composite containing quaternary ammonium dimethacrylate （QADM）, nanoparticles of silver （NAg）, and nanoparticles of amorphous calcium phosphate （NACP）, and （2） to conduct the first investigation of the mechanical properties, biofilm response and acid production vs water-ageing time from 1 day to 12 months. A 4 x 5 design was utilized, with four composites （NACP-QADM composite, NACP-NAg composite, NACP-QADM-NAg composite, and a commercial control composite）, and five water-ageing time periods （1 day, and 3, 6, 9, and 12 months）. After each water- ageing period, the mechanical properties of the resins were measured in a three-point flexure, and antibacterial properties were tested via a dental plaque biofilm model using human saliva as an inoculum. After 12 months of water-ageing, NACP-QADM- NAg had a flexural strength and elastic modulus matching those of the commercial control （P〉 0.1）. Incorporation of QADM or NAg into the NACP composite greatly reduced biofilm viability, metabolic activity and acid production. A composite containing both QADM and NAg possessed a stronger antibacterial capability than one with QADM or NAg alone （P〈0.05）. The anti-biofilm activity was maintained after 12 months of water-ageing and showed no significant decrease with increasing time （P〉0.1）. In conclusion, the NACP-QADM-NAg composite decreased biofilm viability and lactic acid production, while matching the load- bearing capability of a commercial composite. There was no decrease in its antibacterial properties after 1 year of water-ageing. The durable antibacterial and mechanical properties indicate that NACP-QADM-NAg composites may be useful in dental restorations to combat caries.

关 键 词：antibacterial composite calcium phosphate nanoparticles human saliva microcosm biofilm long-term durability quaternaryammonium silver nanoparticles 

分 类 号：R783.1[医药卫生—口腔医学]