RANTES对MPP^+诱导的PC12细胞凋亡的保护作用及其机制研究  

The protective effect and mechanism of RNATES against MPP^+ induced PC12 cellular apoptosis

作　　者：姚婕[1] 赵蓓[2] 姬文涛[1] 马巧亚[1] 

机构地区：[1]西安交通大学第二附属医院(西北医院)老年神经科,西安710004 [2]陕西省人民医院医保办,西安710068

出　　处：《神经解剖学杂志》2016年第5期647-652,共6页Chinese Journal of Neuroanatomy

摘　　要：目的:探讨RANTES对MPP^+诱导的PC12细胞凋亡的保护作用及其机制。方法:利用MPP^+处理PC12细胞建立帕金森细胞模型,并分别转染RANTES siRNA和pcRANTES,MTT法检测细胞增殖,Annexin V-FITC/PI双染法检测细胞凋亡,Western Blot检测总Akt(t-Akt)、磷酸化-Akt(p-Akt)和cleaved caspase-3蛋白表达水平。结果:RANTES siRNA显著抑制RNATES的表达,而pc DNA RANTES成功使RNATES过表达;过表达RNATES可显著上调MPP^+处理的PC12细胞增殖(P<0.05),并抑制MPP^+诱导的PC12细胞凋亡(P<0.05),下调cleasved caspase-3蛋白表达水平(P<0.05)。此外,过表达RANTES可显著促进p-Akt/t-Akt蛋白表达水平(P<0.05),且PI3K/Akt通路抑制剂LY294002显著抑制RNATES对PC细胞增殖的促进作用和凋亡的抑制作用(P<0.05)。结论:RANTES可通过PI3K/Akt信号通路促进MPP^+处理的PC12细胞的增殖并抑制细胞的凋亡。Objective： To observe the protective effect and its mechanism of RANTES against PC12 cell apoptosis induced by 1-methyl-4-phenylpyridinium（ MPP~＋）. Methods： Pakrkinson＇s disease（ PD） cell model was built by addition of MPP~＋into PC12 cell cultures,and RANTES and pc DNA3. 1-RANTES were transfected into PC12 cells. The cell proliferation was measured using MTT assay,cell apoptosis was detected by Annexin V-FITC / PI assay. The protein expression level of total-Akt（ t-Akt）,phosphorylated-Akt（ p-Akt） and cleaved caspase-3 were measured using Western Blot assay. Results： RANTES siRNA significantly silenced the expression of RANTES,while pc DNA3 RANTES remarkably overexpressed RANTES. Overexpression of RANTES remarkably promoted cell proliferation that inhibited by MPP~＋,inhibited cellular apoptosis induced by MPP~＋（ P〈0. 05） and downregulated the cleasved caspase-3 protein expression level（ P〈0. 05）. In addition,RANTES overexpression markedly up-regulated the ratio of p-Akt / t-Akt（ P〈0. 05）,and the PI3 K / Akt inhibitor LY294002 significantly suppressed the effect of RANTED on cellular proliferation and apoptosis. Conclusion： RANTES promotes cellular proliferation and inhibit apoptosis in PC12 cells treated with MPP~＋partly through PI3 K / Akt signaling pathway.

关 键 词：帕金森 细胞凋亡 细胞增殖 RANTES PC12细胞 

分 类 号：R742.5[医药卫生—神经病学与精神病学]