miR-26a对人肝癌细胞系SMMC-7721增殖和迁移的影响  

Influence of miR-26a on proliferation and migration of human hepatic carcinoma cell line SMMC-7721

作　　者：钱建升[1] 李宇[2] 窦建卫[3] 

机构地区：[1]西安交通大学医学院第一附属医院肿瘤放疗科 [2]陕西省人民医院放疗科 [3]西安交通大学药学院,陕西西安710061

出　　处：《基础医学与临床》2016年第10期1335-1340,共6页Basic and Clinical Medicine

基　　金：国家自然科学基金(81573983)

摘　　要：目的观察miR-26a在人肝癌细胞中的表达情况及高表达的miR-26a对SMMC-7721细胞增殖和迁移的影响。方法生物信息学分析miR-26a在肝癌及正常肝组织中表达水平分布;化学合成miR-26a寡聚核苷酸,并行测序确认;利用pc DNA6.2-GW/Em-GFP-miR质粒构建miR-26a真核表达载体;pc DNA6.2-GW/Em-GFP-miR-26a质粒载体瞬时转染SMMC-7721细胞,实时荧光定量PCR检测miR-26a的mRNA表达水平;高表达miR-26a后,CCK8法和划痕实验分别检测SMMC-7721细胞增殖和迁移能力的变化。结果 miR-26a在肝癌组织中表达水平显著低于正常组织(P<0.001);设计的miR-26a序列与寡核苷酸测序结果比对,匹配程度达100%;pc DNA6.2-GW/Em-GFPmiR-26a质粒瞬转后人肝癌SMMC-7721细胞miR-26a的表达量与对照组相比显著增加(P<0.05);miR-26a过表达SMMC-7721细胞的增殖能力与对照组相比均显著下降(P<0.05);miR-26a过表达组细胞迁移速度低于对照组。结论 miR-26a在肝癌组织中低表达,而上调的miR-26a可抑制人肝癌细胞系SMMC-7721的增殖、迁移能力。Objective To observe the expression of miR-26 a in human hepatic carcinoma and the influence of highly expressed miR-26 a on the proliferation and migration of human hepatic carcinoma cell line SMMC-7721.Methods The miR-26 a expression was analyzed by bioinformatic analysis in human hepatic carcinoma and normal hepatic tissue. The miR-26 a oligonucleotide was synthesized and confirmed by sequencing. The miR-26 a eukaryon expression system was constructed by pc DNA6. 2-GW / Em-GFP-miR plasmid. The miR-26 a was transfected into the SMMC-7721 cells transiently by pc DNA6. 2-GW / Em-GFP-miR-15 a plasmid,and quantified by quantitative realtime PCR. The proliferation and migration of SMMC-7721 cell with high-expressed miR-26 a were detected by CCK8 assay and Wound Healing assay,respectively. Results The miR-26 a was remarkable lower in the hepatic carcinoma tissues than in the normal hepatic tissues,the difference was statistically significant（ P〈 0. 0001）. Thesequence of miR-26 a oligonucleotide was matched with the designed sequence in 100%. The miR-26 a expression was increased statistically（ P〈0.05） in SMMC-7721 cell transfected by pc DNA6. 2-GW / Em-GFP-miR plasmid than control groups. The proliferation velocity of SMMC-7721 decreased in highly expressed miR-26 a group（ P〈0.05）. The migration velocity of SMMC-7721 also decreased in highly expressed miR-26 a group compared with control groups（ P〈0.05）. Conclusions miR-26 a is low expressed in hepatic carcinoma tissue. Highly expressed miR-26 a may decrease the capability of proliferation and migration in human hepatic carcinoma cell line SMMC-7721.

关 键 词：miR-26a 肝癌 增殖 迁移 生物信息学分析 

分 类 号：R735.7[医药卫生—肿瘤]