MTSS1基因在膀胱癌中表达下调促进T24细胞侵袭转移  

MTSS1 gene down-regulated in bladder cancer promotes T24 cells invasion

作　　者：郭艳[1] 尚超[2] 张辉[3] 朱莉[1] 

机构地区：[1]中国医科大学附属口腔医院,辽宁省口腔医学重点实验室,沈阳110000 [2]中国医科大学基础医学院神经生物教研室,沈阳110000 [3]中国医科大学附属盛京医院泌尿外科,沈阳110000

出　　处：《解剖科学进展》2016年第5期473-476,共4页Progress of Anatomical Sciences

基　　金：国家自然科学基金青年基金(81301834);辽宁省自然科学基金(2013021094)

摘　　要：目的筛查膀胱浸润性尿路上皮癌差异表达的基因,并探讨MTFSS1基因对膀胱癌T24细胞侵袭能力的影响。方法应用基因芯片结合生物信息学软件筛查膀胱浸润性尿路上皮癌差异表达的基因,并通过荧光定量PCR和Western Blot进行验证;通过转染技术,应用MTT和Transwell小室法分别检测MTSS1基因对膀胱癌T24细胞增殖和侵袭能力的影响。结果膀胱浸润性尿路上皮癌和其癌旁组织表达差异2倍以上的基因有1392个,荧光定量PCR和Westem Blot显示MTSS1在癌组织中较在癌旁组织中表达降低,两者差异35倍,P=0.0065,主要在趋化因子信号转导通路中发挥重要作用。转染结果显示MTSS1基因高表达列T24细胞增殖能力没有影响,而能明显抑制T24细胞的侵袭转移能力(P<0.05)。结论 MTSS1基因在膀胱浸润性尿路上皮癌中表达下调是其促进该肿瘤侵袭转移的重要因素之一。Objective To screen the differential expressed genes of invasive urothelial bladder cancer and to explore the influence of MTSSI gene to T24 cell invasion ability. Methods Gene array together with bioinformatics, quantitative PCR and Western blot were used to screen and verify the differential expressed genes in invasive urothelial bladder cancer. MTT and transwell assay were used to analysis the T24 cells proliferation and invasion abilities following transfection the MTSS1 over-expressed vectors. Results Totally 1392 genes wero differentially expressed in invasive urothelial bladder cancer, Quantitative PCR and Western blot confirmed that MTSS1 gene was down-regulated 3.5 times in tumors than in paracancer tissue（P=0.0065）, played function in chemokine signaling pathway. Transfection results showed that MTSS 1 gene had no effect on T24 cells proliferation, but significantly inhibited T24 cells inwqion. Conclusion MTSS 1 gene down-regulated in invasive urothelial bladder cancer was one of the key factors promoting cancer aggression.

关 键 词：膀胱浸润性尿路上皮癌 基因芯片 MTSS1 细胞增殖 侵袭 

分 类 号：R737.14[医药卫生—肿瘤]