二氯乙酸盐DCA对非小细胞肺癌脑转移瘤作用效果的研究  

The effect of dichloroacetate on brain metastatic tumor cells from non-small cell lung cancer

作　　者：姚冰[1] 喻为红 陈一[1] 史记[1] 张烨[1] 孙佩欣[1] 隋锐[1] 朴浩哲[1] 

机构地区：[1]中国医科大学肿瘤医院,辽宁省肿瘤医院神经外科,辽宁沈阳110042

出　　处：《解剖科学进展》2016年第5期486-489,共4页Progress of Anatomical Sciences

基　　金：辽宁省博士启动基金计划(20131059)

摘　　要：目的探讨二氯乙酸盐(dichloroacetate,DCA)在体外药敏检测实验中对非小细胞肺癌(NSCLC)脑转移瘤细胞的抑制作用。方法使用DCA干预NSCLC脑转移瘤细胞,应用四甲基偶氮唑盐比色法(MTT),观察DCA对肿瘤细胞的抑制情况,对转移瘤细胞抑制率进行检测;应用Western blot实验检测caspase-3、bcl-2的表达变化。结果 MTT实验发现,伴随DCA浓度的线性升高,肿瘤细胞抑制作用明显;Western blot实验发现,伴随DCA浓度的升高,非小细胞肺癌肺癌脑转移瘤细胞caspase-3表达升高,而bcl-2的表达降低。结论 DCA对非小细胞肺癌肺癌脑转移瘤细胞有明显的抑制作用。Objective To study the effect of dichloroacetate（DCA） on brain metastatic tumor cells from non- small cell lung cancer（NSCLC） in in-vitro drug sensitivity experiment. Methods Methyl thiazolyl tetrazolium（MTT） was applied to detect the inhibition effect of DCA on tumor cells, the expressions of caspase-3 and bcl-2 were detected by Western blot method. Results With the increase of DCA concentrations, significant inhibition of tumor cells was observed detected by MTr. With the increase of DCA concentrations, the expression level of caspase-3 was increased significantly, but decreased for the expression of Bcl-2 by Western blot. Conclusion DCA has a significant inhibitory effect on the brain metastasis tumor cells from NSCLC in vitro.

关 键 词：二氯乙酸盐 人肺癌 脑转移瘤 

分 类 号：R739.4[医药卫生—肿瘤]