壳聚糖多孔明胶微球温敏凝胶载药体的建立与评价  

The establishment and evaluation of thermo-sensitive hydrogel drug carrier of chitosan porous gelatin microsphere

作　　者：于湛[1] 于敏[3] 贾宏光[1] 顾湘[1] 张丽川[1] 黄小兵[1] 刘娅[2] 孙建军[2] 魏永祥[1] 

机构地区：[1]首都医科大学附属北京安贞医院耳鼻咽喉头颈外科,北京100029 [2]海军总医院全军耳鼻咽喉中心,北京100048 [3]中国医科大学基础医学院细胞生物学教研室,卫生部细胞生物学重点实验室,沈阳110122

出　　处：《解剖科学进展》2016年第5期490-492,共3页Progress of Anatomical Sciences

基　　金：国家自然科学青年基金(81201169,81201890)

摘　　要：目的 寻找有效的跨圆窗膜载药体,制备多孔明胶微球结合壳聚糖/β-GP溶液的温敏特点,制备新型可注射、可降解、无毒副作用并具有缓释效应的药物载体,为后续动物实验提供研究基础。方法 利用化学反应合成碳酸钙（CaCO_3）模板,采用W/O型乳化-固化法制备了明胶-碳酸钙复合微球,用EDTA处理除去碳酸钙,得到多孔的明胶微球。并以环境扫描电子显微镜对多孔明胶微球进行表征。利用壳聚糖与β-GP合成溶液物理混合形成壳聚糖多孔明胶微球温敏凝胶载药体。结果 扫描电镜显示,明胶微球表面呈多孔结构,粒径在2~4um之间。壳聚糖/β-GP冻干后呈多孔网络状。壳聚糖/β-GP具有温敏性,且两者共混后温敏性不变。结论 壳聚糖/β-GP溶液共混可制备成具有温敏效果的凝胶载药体,为跨膜药物转运的研究提供基础。Objective To search for an effective drug carrier to penetrate the round window membrane, the porous gelatin microsphere combined the thermo-sensitive chitosan/13 -GP gelatin was prepared. Methods CaCO3 template was synthesized chemically, and gelatin CaCO3 microsphere was prepared by W/O emulsive solidified method. Then CaCO3 was removed by EDTA, and porous gelatin microsphere was prepared. Scanning electron microscope was utilized for microsphere characterization. Thermo-sensitive drug carrier of chitosan porous gelatin mierosphere was obtained by physical mixation of chitosan and β -GP. Results The chitosan/β -GP solution was injectable, the diameters of chitosan/β -GP gelatin microspheres were about 2 - 4him with porous network after freezing and thermo sensitivity. Condusion Thermo-sensitive drug carrier of chitosan/β -GP gelatin microspheres would be useful for transmembrane drug delivery.

关 键 词：壳聚糖 明胶 微球 碳酸钙 温敏凝胶 

分 类 号：R914.2[医药卫生—药物化学]