L-氨基丙醇中对映异构体的分离与测定  

Separation and Determination of Enantiomer of L- Alaninol by HPLC

作　　者：杨杰锋 徐川龙 张洁 李会林 

机构地区：[1]浙江省医药经济发展中心,杭州310012 [2]浙江司太立制药股份有限公司,浙江台州317317 [3]浙江省食品药品检验研究院,杭州310052

出　　处：《江西中医药大学学报》2016年第5期93-95,共3页Journal of Jiangxi University of Chinese Medicine

摘　　要：目的：建立高效液相色谱法测定L-氨基丙醇中的对映异构体。方法：采用手性色谱柱Chiralpak IC,以正己烷∶甲醇∶异丙醇（500∶25∶15）为流动相,流速为0.7 m L/min,检测波长为320 nm,柱温25℃。结果：D-氨基丙醇在0.2-1.5μg/m L范围内线性关系良好（r〉0.9990）,精密度及回收率良好（RSD〈2.0%,n=6）,L-氨基丙醇与D-氨基丙醇分离度良好。结论：采用高效液相色谱法,柱前衍生拆分氨基丙醇对映异构体,该方法具有简单、快捷、重复性好等优点,可用于生产过程中L-氨基丙醇对映体的质量检测。Objective ：To establish a HPLC method for the separation and determination of L - alaninol and its enantiomeric. Method： The analysis was carried out on a Chiralpak chiral chromatography column with hexane - methanol - isopropanol mixture （500： 25： 15） as the mobile phase at a flow rate of 0.7 ml /min. The detection wavelength was 320 nm and the column temperature was 25℃. Results： The linear calibration for the L - alaninol was 0.2 - 1.5μg/mL（r 〉0.9990） , the precision and recovery were good （RSD 〈 2 . 0% ,n=6） . Also good resolution of L - alaninol and its R - alaninol was achieved with pre - column derivatization followed by chiral column separation. Conclusion ： Simple operation, good repeatability make it a reliable method for the quality control of L - alaninol enantiomers in the process of production.

关 键 词：高效液相色谱法 L-氨基丙醇 Chiralpak手性柱 对映异构体 

分 类 号：R914.1[医药卫生—药物化学]