电极优化的PCR芯片及其扩增实验  

PCR Chip with Optimized Electrodes and Amplification Experiment

作　　者：陈伟[1] 崔峰[1] 郭兆鑫 陈文元[1] 吴校生[1] 刘武[1] 张卫平[1] 

机构地区：[1]微米纳米加工技术国家级重点实验室上海市北斗导航与位置服务重点实验室上海交通大学微纳电子学系,上海200240

出　　处：《仪表技术与传感器》2016年第8期75-76,80,共3页Instrument Technique and Sensor

基　　金：国家重点基础研究发展计划(973计划)项目(2011CB933102);上海市科委平台建设项目(11DZ2290303)

摘　　要：对传统的蛇形加热电极结构进行了优化,提升PCR芯片反应的温度分布均匀性。设计了一种电极片可重复使用且和反应腔室片可分离的PCR芯片。利用红外热像测温仪对优化前后的电极片进行了温度场分布测量,结果表明优化后的加热电极在反应腔室范围内的温度均匀性小于1℃。与大型PCR仪的扩增实验结果对比表明,该PCR芯片实现了239 bp的DNA片段的高效扩增。The traditional serpentine heating electrode structure was optimized,and uniformity of temperature distribution of PCR chip reaction was improved.A separable PCR chip from chamber with reusable electrodes part was designed.The infrared thermometer was used to measure temperature distribution of the fabricated electrode-chip.The test results show that this kind of PCR chip with optimized electrode pattern can provide uniform temperature distribtion in the scope of the reaction chamber where temperature fluctuation is in 1 ℃.Compared with large PCR amplification experimental results,the PCR chip achieve the efficient amplification of 239 bp DNA fragment.

关 键 词：MEMS PCR芯片 PDMS 温度场 扩增 

分 类 号：TP274[自动化与计算机技术—检测技术与自动化装置]