乳腺癌患者绝经前后E2/ER表达变化及其与肿瘤细胞FasL表达的相关性  

E2/ER change and its relation with FasL expression in breast cancer patients before and after menopause

作　　者：刘玉[1] 高炳玉[1] 夏立平[1] 吴毓优[1] 王昱[1] 王超群[1] 

机构地区：[1]海南医学院肿瘤研究所,海南海口570102

出　　处：《海南医学院学报》2016年第20期2448-2450,2454,共4页Journal of Hainan Medical University

基　　金：2016年度海南省科技厅科技合作项目(ZDYF2016224)~~

摘　　要：目的:探讨乳腺癌患者绝经前后外周血和瘤体组织E2/ER的变化与肿瘤细胞凋亡相关因子配体FasL基因表达的相关性。方法:分别选取预行外科切除术的绝经前乳腺癌患者21例和绝经后乳腺癌患者19例,术前抽取外周空腹静脉血,以ELISA试剂盒测定血清E2与ER的含量,并于术后保留瘤体和瘤周正常组织病理切片,以免疫荧光法观察E2、ER、及FasL的表达。结果:ELISA检测结果显示,绝经后患者外周血E2、ER水平显著低于绝经期患者(P<0.05),但瘤体组织E2、ER水平与绝经前患者无显著差异性(P>0.05)。免疫荧光检测结果显示,绝经前、后患者ER的阳性表达率分别为52.4%、57.9%,两者相比无显著差异(P>0.05);绝经前后ER阳性乳腺癌患者的瘤体组织FasL基因表达趋势均与E2、ER一致,且呈直线正相关趋势(P<0.05),ER阴性乳腺癌患者瘤体组织FasL基因表达趋势虽与E2、ER一致,但无显著线性相关性(P>0.05)。结论:乳腺癌患者绝经后外周血E2、ER水平有所下降,但肿瘤组织内仍可保留高水平,且肿瘤组织ER高水平介导的FasL高表达可能为绝经前后患者免疫逃逸的重要机制之一,可作为乳腺癌尤其是ER阳性乳腺癌患者临床治疗的重要靶点进行深入研究。Objective：To explore the E2/ER changes both in the peripheral blood and tumors,as well as its effect on factor-related apoptosis ligand（FasL）gene expression of breast cancer patients before and after menopause.Methods：Premenopausal（n=21）and postmenopausal（n=19）breast cancer patients were enrolled in this study for a comparative analyse.Peripheral blood was extracted to detect the E2 and ER concentration by ELISA kit.At the same time,pathological slices of tumor and healthy tissue besides the tumor were both prepared to detect the E2,ER and FasL expression with immunofluores-cence.Results：We found that postmenopausal patients had significantly lower peripheral levels of E2 and ER than premenopausal patients,while tumor levels of E2 and ER had no significant difference between the two groups.Further more,ER positive expression rate of patients before and after menopause was respectively 52.4% and 57.9% showing no significant differences between the two groups;and FasL gene expression had linear positive correlation with tumor E2 and ER expression both in patients with ER positive expression before and after menopause.It was shown that E2 and ER level tumor tissues could keep high level independent of environment which contributes to the progress of breast caner;and high dose E2 and ER level of the tumor tissues which up regulated the expression of FasL may be an important factor for immune escape.

关 键 词：乳腺癌 绝经前后 E2 ER FASL表达 

分 类 号：R737.9[医药卫生—肿瘤] R392.32[医药卫生—临床医学]