人工栽培蛹虫草致病木霉CCBH-M2的鉴定及生物学特性  

Identification of Pathogenic Trichoderma CCBH-M2 from Cultivated Cordyceps militaris and Its Biological Characteristics

作　　者：李娟[1,2] 刘晓红[1,2] 宋洁[1,2] 李莉[1] 

机构地区：[1]辽东学院农学院,辽宁丹东118003 [2]辽东生物产业研究所,辽宁丹东118003

出　　处：《西南农业学报》2016年第9期2216-2220,共5页Southwest China Journal of Agricultural Sciences

基　　金：辽宁省教育厅科学研究一般项目(L2012472);丹东市科学技术计划项目(农业公关及产业化)(201402)

摘　　要：自人工栽培蛹虫草培养料中分离得到1株致病木霉CCBH-M2,通过对其进行形态学鉴定及ITS序列系统发育分析,确定该木霉菌株为钩状木霉Trichoderma hamatum（Bon.）Bain.。实验对该致病菌进行了生物学特性研究,结果表明,温度、p H值及培养料含水量对该钩状木霉生长影响显著（P〈0.05）,其最适生长温度为26℃,最适p H值为6.0,培养料最适含水量为50%～60%。该研究结果是钩状木霉对人工栽培蛹虫草具有致病性的首次报道。A pathogenic Trichoderma strain CCBH-M2 was isolated from culture material of cultivated Cordyceps militaris. The morphological characteristics and phylogenetic analysis showed that strain CCBH-M2 was Trichoderma hamatum（ Bon.） Bain. The biological characteristics of T. hamatum were studied and the results showed that temperature,p H value and water content of the culture medium significantly influenced the growth of strain CCBH-M2（ P 0. 05）,and its optimum growth temperature was 26 ℃,the optimum p H value was 6. 0 and the optimum water content of culture materials was 50 %- 60 %. The result was the first report of T. hamatum pathogenicity on cultivated C.militaris.

关 键 词：蛹虫草 致病木霉 钩状木霉 生物学特性 

分 类 号：S546.9[农业科学—作物学]