2014年广州市4株1型登革病毒的分子流行病学分析  

Molecular epidemiolgy of dengue outbreak in Guangzhou in 2014 causing by serotype 1 dengue virus

作　　者：陈月[1] 杨柳[1] 张培[1] 严华成[1] 任瑞文[1] 

机构地区：[1]广州军区疾病预防控制中心,广东广州510507

出　　处：《热带医学杂志》2016年第9期1184-1187,共4页Journal of Tropical Medicine

基　　金：广东省科技计划项目资助(2014A020212252;2014A020219007;2015A020218003;2016A020219006)

摘　　要：目的探讨2014年广州4株1型登革病毒分离株基因型及来源。方法 4株2014年广州市1型登革病毒分离株提取RNA,逆转录PCR扩增包膜蛋白基因并测序,对其进行系统进化分析,结合流行病学资料进行生物信息学分析。结果 4株2014年1型登革病毒分离株分别来自不同的基因亚型,即基因I型(1株)和基因V型(3株),同广州或广东其他地区2013年分离株高度相似,最初由东南亚或印度输入我国。结论广州市2014年1型登革病毒为由商贸旅游由外地输入到广东地区,并已逐渐本地化,须进一步从蚊媒中证实。Objective The genetic relationships among four dengue virus serotype 1（DENV-1） isolates from the Guangzhou dengue epidemic of 2014 were studied by sequence analysis of the envelope（E） genes. Methods The whole length of E gene from four DENV-1 isolates was amplified by reverse transcriptase-polymerase chain reaction（RT-PCR）and then sequenced.The phylogenetic tree was drawn by neighbor-joining method.The bioinformatics analysis was performed by combining the phylogenetic information and the epidemiology data. Results Among 4 isolates revealed an epidemic strain in the outbreak.Phylogenetic study demonstrated that the 2014 Guangzhou isolates were of genotype I and genotype V and similar from Guangdong DENV-isolates from 2013,which were initially imported from Southeast Asia and India. Conclusion Our result showed the same genotype in Guangdong isolates of 2013 and 2014 suggested the 2014 Guangzhou DENV-1 isolated strains were imported from nearby countries by trade and tourism and gradually autochthonous; further prove of their origins was needed.

关 键 词：1型登革病毒 系统进化分析 包膜蛋白 

分 类 号：R512.8[医药卫生—内科学]