2014年深圳市龙岗区诺如病毒胃肠炎暴发的分子流行病学特征  

Molecular epidemiological characteristics of norovirus gastroenteritis outbreaks in Longgang district of Shenzhen in 2014

作　　者：杨慧[1] 李静媚[1] 陈应坚[1] 吴滨[1] 林琳[1] 周健明[1] 

机构地区：[1]深圳市龙岗区疾病预防控制中心,广东深圳518172

出　　处：《热带医学杂志》2016年第9期1208-1211,共4页Journal of Tropical Medicine

摘　　要：目的了解2014年深圳市龙岗区诺如病毒胃肠炎暴发疫情的分子流行病学特征。方法收集监测期间病毒性胃肠炎暴发疫情患者的肛拭子样本,采用实时荧光定量RT-PCR方法检测诺如病毒核酸,并选择部分阳性样本扩增VP1基因区,阳性产物回收并测序分析。结果 13起暴发疫情共检测标本157份,诺如病毒GⅡ型阳性25份,阳性率为15.9%,未检出诺如病毒GⅠ型。测序获得来自7起疫情的GⅡ型诺如病毒VP1基因序列,5株为GⅡ.4亚型,为新型变异株GII.4_Sydney 2012,2株GⅡ.17亚型,与2014年深圳本地株(KP718662)亲缘关系最近。结论 2014年深圳市龙岗区诺如病毒腹泻暴发的主要型别为GⅡ,并以GⅡ.4为主,流行优势株为GII.4_Sydney 2012。Objective To investigate the molecular epidemiological characteristics of norovirus gastroenteritis outbreaks in Longgang district of 2014. Methods Anal swab specimens were collected during the monitoring of viral gastroenteritis outbreak. Norovirus was detected by real-time quantitative RT-PCR. VP1 gene of norovirus was amplified and sequenced.Results Viral nucleic acid of norovirus was detected in 157 anal swab specimens of 13 outbreaks. The positive rate of norovirus G Ⅱ was 15.9% and norovirus G Ⅰ was not observed. The VP1 sequencing results of norovirus G Ⅱ in 7outbreaks showed that 5 strains were belonged to new variants（Sydney 2012） of genotype GII.4 and 2 strains were genotype GII.17,and was close to the G Ⅱ.17（KP718662） variant. Conclusions The main genogroups of norovirus in Longgang district were GII. GII.4 was one of the most major genotype of norovirus,while GII.4_Sydney 2012 variant was identified as the predominant strain in Longgang district during 2014.

关 键 词：诺如病毒 序列分析 系统进化树 

分 类 号：R512.5[医药卫生—内科学]