调节因子X5在消化系统肿瘤中高表达  

Overexpression of regulatory factor X5 in digestive system neoplasms

作　　者：赵杨静[1,2] 谢兴旺[2] 张恒辉[2] 曹慧[3] 魏来[2] 陈红松[2] 邵启祥[1] 

机构地区：[1]江苏大学医学院,江苏镇江212013 [2]北京大学人民医院北京大学肝病研究所,北京100044 [3]浙江大学附属第一医院病理科,浙江杭州310003

出　　处：《江苏大学学报（医学版）》2016年第2期168-171,共4页Journal of Jiangsu University：Medicine Edition

基　　金：国家自然科学基金资助项目(81372221;81201569;81541151);江苏省临床医学科技专项(BL2013024)

摘　　要：目的:观察调节因子X5(regulatory factor X5,RFX5)在消化系统肿瘤组织中的表达水平。方法:通过分析美国癌症和肿瘤基因图谱计划(the Cancer Genome Atlas,TCGA)中常见消化系统肿瘤的RNA测序数据,获得RFX5 mRNA在癌组织和癌旁组织的表达水平。利用免疫组织化学方法检测25例癌组织和配对的癌旁多肿瘤组织芯片中食管鳞状细胞癌、胃腺癌、结肠腺癌、直肠腺癌、肝细胞肝癌和胰腺腺癌的RFX5蛋白表达水平。结果:初步研究显示,上述6种消化系统肿瘤中RFX5 mRNA和蛋白水均明显高于癌旁组织,且在胃腺癌和肝细胞肝癌中的表达上调最为显著。结论:RFX5 mRNA和蛋白在常见消化系统癌组织中均呈过表达。Objective ： To investigate the expression levels of regulatory factor X5 （ RFX5 ） in digestive system neoplasms. Methods： RFX5 mRNA expression levels were determined by analyzing the RNAseq data from the the Cancer Genome Atlas（TCGA） database. By using tissue microarray technology and immunohisto- chemical staining, RFX5 protein expression was confirmed in six different types of digestive system tumors （e- sophageal, stomach, colon, rectal, liver and pancreatic cancers） and corresponding non-tumor normal tis- sues. Results： RbX5 mRNA and protein levels were upregulated in six malignancies, and the altitudes in- crease more significantly in stomach adenocarcinoma and hepatoccllular carcinoma than other cancer types. Conclusion： Expressions of RFX5 mRNA and protein were both elevated in digestive system neoplasms.

关 键 词：调节因子X5 消化系统肿瘤 过表达 组织芯片 

分 类 号：R735[医药卫生—肿瘤]