变异链球菌转肽酶A基因型与乳牙高龋的相关性  

The correlation between the sortase A genotype of Streptococcus mutans and high-severity childhood caries

作　　者：于丽霞[1] 庄沛林[1,2] 林焕彩[1] 

机构地区：[1]中山大学光华口腔医学院·附属口腔医院,广东省口腔医学重点实验室,广州510055 [2]中山大学孙逸仙纪念医院口腔科,广州510120

出　　处：《中华口腔医学研究杂志（电子版）》2016年第4期238-243,共6页Chinese Journal of Stomatological Research(Electronic Edition)

基　　金：国家自然科学基金(81271123)

摘　　要：目的在控制乳牙龋危险指标的前提下,探讨乳牙高龋与变异链球菌(简称变链菌)转肽酶A(srt A)基因型的关系。方法采用ABI Variant Reporter软件对121株分离于高龋儿童的变链菌和121株分离于无龋儿童的变链菌srt A基因测序结果进行基因型分析。采用单因素分析方法分析srt A基因型数量、srt A各基因型以及社会、行为变量在两组中的频率分布情况,采用多因素Logistic回归方法控制混杂因素,分析变链菌srt A基因型与乳牙高龋的相关性。结果共发现15种基因型,其中无龋组中9种,高龋组中11种。3A基因型在无龋组中的突变率高于高龋组(P=0.033),3E基因型在高龋组中的突变率高于无龋组(P=0.037)。多因素分析结果表明:高月龄、低出生体重、过长的母乳喂养时间、高固体糖摄入频率、较高的可视菌斑百分率、较少的srt A 3A基因型与乳牙高龋相关。结论在有变链菌定植的儿童中,变链菌srt A 3A基因型与乳牙龋易感性相关。此外,月龄、出生体重、母乳持续喂养时间、固体糖摄入频率、可视菌斑等因素也参与早期儿童龋的发生、发展。Objectives To make a primary research on the relationship between genotypes of sortase A gene（srtA）of Streptococcus mutans（S.mutans）and highseverity childhood caries on the base of existenceof no other factors related to childhood caries. Methods The ABI Variant Reporter software was used to analyze the genotypes of the srtA gene of 121 S.mutans strains isolated from the highseverity caries children and 121 S.mutans strains isolated from the cariesfree children. Bivariate analysis was used to analyze the srtA genotype distribution and the frequencies of social and behavioural variants between groups. Multiple logistic regression models were used to assess the relationship between the srtA genotype and childhood caries after controlling confounding factors. Results A total of 15 genotypes were found：9 in caries free group and 11 in highseverity caries group. The frequency of 3A genotype was significantly higher in caries free group than highseverity caries group（P=0.033）. The frequency of 3E genotype was significantly higher in highseverity caries group than caries free group（P=0.037）. The bestfitting model showed that older age（months）,low weight at birth,high frequencies of solid sugar consumption,prolonged breastfeeding,a high proportion of visible plaque,and 3A genotype of the srtA gene of S.mutans were associated with high severity caries in children. Conclusions The present study suggested that the 3A genotype of the srtA gene of S.mutans may correlate with caries susceptibility in children. In addition,age, weight at birth,duration of breastfeeding,solid sugar consumption,and poor oral hygiene contributed to primary childhood caries.

关 键 词：龋病 变异链球菌 转肽酶A 基因型 

分 类 号：R788.1[医药卫生—口腔医学]