非酒精性脂肪性肝炎患者血清CK-18 M30水平测定的意义  

Clinical value of Serum CK-18 M30 levels in patients with nonalcoholic steatohepatitis

作　　者：齐凤祥[1] 江勇[1] 张颖[1] 宓余强[2] 徐亮[2] 宋国栋[3] QI Feng-xiang JIANG Yong ZHANG Ying MI Yu-qiang XU Liang SONG Guo-dong(Department of Gastroenterology, the Second Hospital of Tianjin Medical University, Tionjin 300211, China)

机构地区：[1]天津医科大学第二医院消化科,300211 [2]天津市第二人民医院肝内科,300192 [3]天津医科大学第二医院普外科,300211

出　　处：《实用医学杂志》2016年第18期3019-3022,共4页The Journal of Practical Medicine

基　　金：天津市卫生局科技基金项目(编号:2015KZ093);天津市卫生局科技基金攻关项目(编号:12KG119);天津市卫生和计划生育委员会中医中西医结合科研课题(编号:2015061)

摘　　要：目的:检测非酒精性脂肪性肝炎(NASH)患者血清细胞角蛋白片段(CK-18 M30)的水平,探讨其表达与NASH之间的关系。方法:收集33例健康对照者、24例非酒精性单纯性脂肪肝(NAFL)患者、21例NASH患者空腹静脉血,检测所有对象的CK-18 M30及丙氨酸转氨(ALT)、天门冬氨酸转氨酶(AST)、γ-谷氨酰胺转肽酶(GGT)并对所有NAFL、NASH患者行肝组织活检,对活检组织行NAFLD活动度积分(NAS),统计分析上述指标组间的差异,Pearson相关分析NASH患者血清CK-18 M30与NAS及血清中ALT、AST、GGT的相关性。结果 :健康对照组、NAFL组、NASH组血清中CK-18 M30的含量分别是(96.557 2±41.2268)U/L、(104.321 7±45.167 3)U/L、(263.125 5±61.578 1)U/L,NASH组血清中CK-18 M30较健康对照组、NAFL组均明显增高,差异有统计学意义;NASH患者血清CK-18 M30与NAS及血清ALT成正相关性,r值分别为0.601 5、0.420 6。结论:NASH患者血清中的CK-18 M30明显增高,检测血清CK-18 M30的含量对NASH的诊断有一定的意义。Objective To detect the level of serum fragmented cytokeratin 18 （CK-18 M30） in patients with nonalcoholic steatohepatitis （NASH）, to explore the relationship between the expression of CK-18 M30 and NASH. Methods 33 healthy people as control group, 24 nonalcoholic simple fatty liver （NAFL） patients, and 21 NASH patients were included in this study. CK-18 M30, ALT, AST and GGT were detected in all patients＇ vein blood. NAFLD activity points （NAS） was examined in biopsy specimens of NAFL patients and NASH patients. Pearson correlation was applied to analyze the correlations between serum CK-18 M30, ALT, AST, GGT and the NAS of liver tissue in NASH group. Results Serum CK-18 M30 level of healthy control, NAFL and NASH group were （96.557 2±41.226 8）U/L, （104.321 7±45.167 3）U/L, （263.125 5±61.578 1）U/L respectively. Serum CK-18 M30 level in NASH patients positively correlated with both NAS of liver tissue and serum ALT, which correlation coefficient r values were 0.601 5 and 0.420 6. Conclusion The concentration of serum CK-18 M30 could be used as a marker in the diagnosis of NASH.

关 键 词：非酒精性脂肪性肝炎 血清细胞角蛋白片段(CK-18 M30) 非酒精性单纯性脂肪肝 

分 类 号：R575.5[医药卫生—消化系统]