谷氨酸棒状杆菌发酵生产L-瓜氨酸及发酵条件优化  

Optimization of L-citrulline production by Corynebacterium glutamicum

作　　者：丁叶[1] 郝宁[1] 胡洁[1] 谭玉岩 郭格格 

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京211800

出　　处：《生物加工过程》2016年第5期6-11,共6页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金(21406113);江苏省自然科学基金(BK20140932);江苏省产学研前瞻性联合研究项目(BY2014005-07)

摘　　要：以代谢控制发酵理论为指导,重点对C.glutamicum 366菌株进行摇瓶发酵条件的优化。应用响应面法优化发酵培养基的配比,优化后的发酵培养基:葡萄糖63.33 g/L、精氨酸196.96 mg/L、(NH4)2SO445.79 g/L、生物素35.72μg/L、K2HPO4·3H2O 1.0 g/L、KH2PO41.0 g/L、Mg SO4·7H2O、0.25 g/L、Mn SO4·H2O 0.02 g/L、Fe SO4·7H2O 0.02g/L、Zn Cl21 mg/L、Cu SO40.2 mg/L、VB1200μg/L、Ca CO330 g/L。摇瓶发酵培养条件:温度30℃、摇床转速200r/min、初始p H 7.0。在此发酵条件下,菌株进行摇瓶发酵72 h,产L-瓜氨酸14.96 g/L,相比优化之前提高了75.8%。We optimized the fermentation for L-citrulline production by C. glutamicum 366 strain. Mediam was optimized by response surface analysis Design-Expert software. The fermentation conditions were also studied. The optimum medium was： glucose 63. 33 g / L,arginine 196. 96 mg / L,ammonium sulfate 45. 79 g / L,biotin 35. 72 μg / L,K2HPO4·3H2O 1. 0 g / L,KH2PO41. 0 g / L,Mg SO4·7H2O 0. 25 g / L,Mn SO4·H2O 0. 02 g / L,Fe SO4·7H2O 0. 02 g / L,Zn Cl21 mg / L,Cu SO40. 2 mg / L,vitamin B1200 μg / L and Ca CO330 g / L.The optimum fermentation conditions were： temperature at 30 ℃,the rotate speed of 200 r / min,initial p H 7. 0.Under this condition,the production of L-citrulline was 14. 96 g / L after 72 h shake flask fermentation,increased by 75. 8% compared to the control.

关 键 词：谷氨酸棒状杆菌 瓜氨酸 发酵 

分 类 号：TQ464.7[化学工程—制药化工]