结直肠癌相关肿瘤标志物的定量蛋白质组学研究  

Quantitative proteome analysis of tumor markers associated with colorectal cancer

作　　者：张彦斌[1] 刘月[1] 叶颖江[2] 张辉[2] 黄红艳[1] 李莎[1] 王杉[2] 任军[1] 

机构地区：[1]首都医科大学附属北京世纪坛医院肿瘤研究室,100038 [2]北京大学人民医院胃肠外科

出　　处：《中华普通外科杂志》2016年第10期839-842,共4页Chinese Journal of General Surgery

基　　金：北京市博士后工作经费资助项目（2015422-54）

摘　　要：目的 筛选结直肠癌相关的肿瘤标志物.方法 收集20例结直肠癌组织及配对的正常结肠黏膜上皮组织,分别采用激光捕获显微切割技术切割纯化结直肠癌细胞和正常结肠黏膜上皮细胞;应用基于生物质谱的乙酸酐稳定同位素标记定量蛋白质组学方法分析鉴定结直肠癌和正常结肠黏膜之间差异表达的蛋白质;借助生物信息学工具对差异蛋白进行功能聚类分析.结果 共鉴定得到137个在结直肠癌和正常结肠黏膜之间差异表达的蛋白质,其中67个蛋白在结直肠癌中的表达上调,70个蛋白在结直肠癌中的表达下调.对差异蛋白进行功能通路分析,得到了7个高可信的功能网络,包括细胞生长增殖、氨基酸代谢、炎症反应、胚胎发育、细胞分化、分子转运及细胞骨架的构成.结论 基于生物质谱的乙酸酐稳定同位素标记定量蛋白质组学方法是筛选结直肠癌肿瘤标志物的有效策略.Objective To screen for colorectal cancer associated tumor markers.Methods Laser capture microdissection （LCM） was used to obtain interested cells from 20 colorectal cancer （CRC） and paired normal mucosa tissues.The differential proteins between the microdissected tumor cells and normal mucosa epithelia were analyzed by acetylation stable isotope labeling coupled with fourier-transform ion cyclotron resonance mass spectrometer （FT-ICR-MS）.Bioinformatics tools were used for cluster analysis of the differential proteins.Results 137 proteins were differentially expressed by at least 2-fold,including 67 proteins up-regulated and 70 down-regulated in cancer.According to IPA（ingenuity pathway analysis）of the differential proteins,7 reliable functional networks including cell growth and proliferation,amino acid metabolism,inflammatory response,embryonic development,cell differentiation,molecular transport,and cytoskeletal components were obtained.Conclusions The strategy combining LCM,stable isotope labeling analysis and LTQ-FT MS was effective to profile proteomic changes in CRC cells.

关 键 词：结直肠肿瘤 蛋白质组学 肿瘤标记 生物学 显微切割 

分 类 号：R735.34[医药卫生—肿瘤]