微小RNA-451在胃癌组织中的表达及其意义  

The study on microRNA-451 expression and its association with gastric cancer

作　　者：荣忠厚[1] 苏忠学[1] 耿文茂[1] 咸国哲[1] 王志意[1] 吴亚光[1] 秦成坤[1] 

机构地区：[1]山东大学附属省立医院肝胆外科,济南250021

出　　处：《中华普通外科杂志》2016年第10期847-849,共3页Chinese Journal of General Surgery

基　　金：山东省科技厅科技发展计划资助项目（2013GSF11862）

摘　　要：目的 研究微小RNA-451（microRNA-451,miR-451）的表达与胃癌的关系.方法 应用实时定量聚合酶链反应检测胃癌及癌旁组织中miR-451的表达,分析miR-451表达与胃癌患者临床病理因素及预后的关系.应用慢病毒LV-mir-451感染人胃癌细胞株BGC-823、MKN-45,建立稳定高表达miR-451的细胞株,检测其增殖、迁移及侵袭能力.结果 胃癌组织中miR-451表达水平比癌旁组织降低（0.010±0.007比0.014±0.008,t=3.015,P＜0.05）,且其下调与淋巴结转移、临床分期及患者生存时间缩短呈正相关.BGC-823和MKN-45细胞内miR-451的高表达对细胞增殖无影响,但降低了BGC-823细胞的迁移率（0.863±0.026比1.107±0.032,t=9.820,P＜0.05;0.863±0.026比1.060±0.054,t=4.369,P＜0.05）和侵袭率（1.040±0.055比1.522±0.001,t=5.84,P＜0.05;1.040±0.055比1.696±0.078,t=10.16,P＜0.05）.结论 miR-451可能作为一种新的判断胃癌预后的标志物.Objective To investigate the relations between microRNA-451 （miR-451） expression and gastric cancer （GC）.Methods Expression of miR-451 in tumor and paired non-cancerous tissues was measured by real-time quantitative polymerase chain reaction.The association of miR-451 expression with clinical pathological characteristics and prognosis was statistically analyzed;in addition,slow virus LV-mir-451 was applied to infect human gastric cancer cell line BGC-823 and MKN-45,and construct miR-451 over-expressed stable cell lines.Finally,to detect cell proliferation,migration and invasion.Results miR-451 expression decreased in GC tissues vs.pericarcinous tissues （0.010 ± 0.007 vs.0.014 ± 0.008,t =3.015,P 〈 0.05）,and its down-regulation positively correlated with lymphatic metastasis,clinical staging and shorter overall survival.miR-451 expression in BGC-823 and MKN-45 cells did not impact on cell proliferation,but did reduce migration rates （0.863 ±0.026 vs.1.107 ±0.032,t =9.820,P〈0.05;0.863 ±0.026 vs.1.060±0.054,t =4.369,P〈0.05） and invasion rates （1.040 ± 0.055 vs.1.522 ± 0.001,t =5.84,P 〈 0.05;1.040 ± 0.055 vs.1.696 ± 0.078,t =10.16,P 〈 0.05） in BGC-823 cells.Conclusions miR-451 may act as a prognostic marker in GC.

关 键 词：胃肿瘤 肿瘤标记 生物学 

分 类 号：R735.2[医药卫生—肿瘤]