ANRIL在肝细胞癌中的表达及与预后的关系  

Expression and prognostic significance of ANRIL in hepatocellular carcinoma

作　　者：丁蔚 谭玉林[1] 王一波[1] 朱小俊 徐逸昕[1] 徐学忠[1] 

机构地区：[1]江苏大学附属武进医院肝胆外科,常州213002

出　　处：《中华普通外科杂志》2016年第10期850-853,共4页Chinese Journal of General Surgery

基　　金：常州市武进区科技发展计划基金资助项目（WS201515）

摘　　要：目的 探讨INK4位点反义非编码RNA （antisense non-coding RNA in the INK4 locus, ANRIL）在肝细胞肝癌组织中的表达与肝癌患者临床病理因素和预后的关系.方法 通过实时定量聚合酶链反应（quantitative real-time PCR,qRT-PCR）确定ANRIL在90例肝癌组织和癌旁肝组织中的表达及与临床病理资料的关系;Kaplan-Meier曲线和多变量Cox比例模型研究与肝癌临床预后相关的影响因素;细胞增殖实验、迁移及侵袭实验检测ANRIL表达对肝癌细胞的影响.结果 ANRIL在肝癌组织中的表达量增加,差异有统计学意义（t=13.083,P＜0.05）;且ANRIL高表达与肝癌TNM分期（r=43.245,P＜0.05）及病理分级（x2=40.724,P＜0.05）密切相关.ANRIL高表达组患者术后平均生存时间为18.2个月（95％ CI：14.9～21.5个月）明显短于ANRIL低表达组患者39.4个月（95％ CI：35.5 ～43.4个月）,差异有统计学意义（x2=47.590,P＜0.05）.多变量分析表明,ANRIL高表达是预后不良的独立预测指标（HR =2.143,95％CI：1.083～4.243,P＜0.05）,差异有统计学意义.体外实验显示ANRIL表达下降可抑制肝癌细胞增殖、迁移及侵袭能力. 结论 ANRIL表达水平是肝癌患者的独立预后影响因素.Objective To investigate the expression level of antisense non-coding RNA in the INK4 locus （ANRIL） in hepatocellular carcinoma （HCC） tissues and to evaluate its relation to clinicopathological features and prognosis of HCC.Methods Quantitative real-time polymerase chain reaction （qRT-PCR） method was used to detect the expression of ANRIL in HCC tissues and adiacent tissues （n =90） and to analyze its relationship with clinicopathological data.Kaplan-Meier curves and multivariate Cox proportional models were used to study the impact on clinical outcome.Small interfering RNA （siRNA） was used to silence ANRIL and to explore the effects of reduced ANRIL expression on cell growth and metastasis.Results ANRIL expression in HCC tissues was significantly higher than in the adjacent non-tumor tissues （t =13.083,P 〈 0.05）.The expression of ANRIL was remarkably associated with the histologic grade （x2 =40.724,P 〈 0.05） and TNM stage （x2 =43.245,P 〈 0.05）.The mean survival time of the patients with high ANRIL was 18.2 months （95% CI：14.9-21.5 months）,shorter than 39.4 months （95% CI：35.5-43.4 months） in low expression （x2 =47.590,P 〈0.05）.Multivariate analysis suggested that high ANRIL expression was an independent predictor of poor prognosis （HR =2.143,95% CI：1.083-4.243,P 〈 0.05）.Decreased expression of ANRIL could suppress the cell proliferation,migration and invasion of HCC cells.Conclusion Positive ANRIL expression is negatively correlated with the prognosis of HCC patients.

关 键 词：癌 肝细胞 

分 类 号：R735.7[医药卫生—肿瘤]