人胰腺癌组织块小鼠皮下移植瘤模型的构建及生物学行为的观察  

Subcutaneously transplanted human pancreatic cancer tissue fragments in a mouse model

作　　者：吴蔚[1] 肖俊[2] 陈琦军[2] 杨冲[2] 钦琦[2] 吴河水[2] 

机构地区：[1]山西医科大学第一临床医学院,太原030600 [2]华中科技大学同济医学院附属协和医院胰腺病研究所

出　　处：《中华普通外科杂志》2016年第10期859-862,共4页Chinese Journal of General Surgery

基　　金：卫生部行业科研专项资助项目（201202007）;山西医科大学大学生创新创业训练资助项目（2014126）

摘　　要：目的 构建来源于人胰腺癌组织块的小鼠皮下移植瘤模型.方法 移植术中获取的人胰腺癌组织块到NOD/SCID小鼠皮下构建移植瘤模型,观察肿瘤生长并进行小鼠体内传代扩增,比较各代移植瘤生长速度,组织HE染色及免疫组化检测Ki67和VEGF表达情况的差异.结果 收集13例胰腺癌组织块标本及6例活检组织标本,其中5例组织块标本移植成功,1例已传至第四代小鼠,4例传至第二代小鼠,随着传代次数的增加,肿瘤生长速度明显加快;HE染色显示移植瘤与其来源的人胰腺癌组织病理形态基本一致;免疫组化染色显示Ki67和VEGF表达增加.结论 通过直接移植人胰腺癌组织块,可成功构建胰腺癌小鼠皮下移植瘤模型,并且移植瘤的变性程度及侵袭性可能随传代增加.Objective To establish a mouse model by transplanting subcutaneously human pancreatic cancer tissue fragments.Methods Surgically resected pancreatic cancer tissue fragments from patients were transplanted into NOD/SCID mice subcutaneously,and then the growth of the tumor and transplanting it into the next generation were observed.The growth rate,HE staining and immunohistochemistry staining of Ki67 and VEGF were compared.Results We have obtained 13 cases ofpancreatic cancer tissues and 6 cases of biopsy specimens.In 5 cases transplantation was successful,in onemouse model passing to fourth generation,in 4 models to second generation.With the increase of generaions,tumor growth accelerated.HE staining showed later passage cells behavior in an identical manner as the primary cells.Immunohistochemistry staining showed that expressions of Ki67 and VEGF are increasing.Conclusions Through transplanting human pancreatic cancer tissue fragments directly,we have constructed mouse model of pancreatic cancer successfully.With the passage of subculture,the malignant degree and invasiveness may increase.

关 键 词：胰腺肿瘤 模型 动物 皮下移植瘤 

分 类 号：R735.9[医药卫生—肿瘤] R-332[医药卫生—临床医学]