药物制剂中咖啡因的拟反相色谱法测定  

Determination of Caffeine in Pharmaceutical Preparations by Per Aqueous Liquid Chromatography

作　　者：董丽丽[1] 李敏[1] 于玲[1] 梁慧锋[1] 

机构地区：[1]邢台学院化学工程与生物技术学院,河北邢台054001

出　　处：《中国医药工业杂志》2016年第10期1301-1303,共3页Chinese Journal of Pharmaceuticals

摘　　要：建立了拟反相色谱法测定药物制剂中的咖啡因。以极性硅胶柱为固定相,以乙腈∶0.03 mol/L甲酸钠溶液（pH 2.9）（5∶95）为流动相,检测波长274 nm,5 min内即可完成样品测定。咖啡因在0.2-107μg/ml范围内线性关系良好,平均回收率为99.3%,RSD为1.66%。A per aqueous phase liquid chromatography method was established for the determination of caffeine in pharmaceutical preparations. A bare silica column was used, with the mobile phase of acetonitrile∶0.03 mol/L sodium formate solution （pH 2.9）（5∶95）, at the detection wavelength of 274 nm. Caffeine could be determined within 5 min. It was linear for caffeine in the range of 0.2—107 μg/ml. The average recovery was 99.3%, with RSD of 1.66%.

关 键 词：咖啡因 拟反相色谱法 含量测定 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.72[化学工程—制药化工]