一株假单胞菌(Pseudomonas)SYBS01降解石油的特性  

Oil degradation characteristics of Pseudomonas SYBS01

作　　者：于彩虹[1] 赵粉红[1] 吴东奎[1] 丁燕燕[1] 董聿森 朱晓晶[1] 

机构地区：[1]中国矿业大学(北京)化学与环境工程学院,北京100083

出　　处：《环境工程学报》2016年第10期6042-6048,共7页Chinese Journal of Environmental Engineering

基　　金：国家高技术研究发展计划(863)项目(2007AA10Z404);教育部高校青年业务基金(2013QH11);大学生创新实验项目(201411413026)

摘　　要：从石油污染土壤中筛选得到一株石油降解菌SYBS01,鉴定为假单胞菌(Pseudomonas sp.)。通过单因素实验和正交实验进行石油降解条件优化,结果为35℃、170 r·min^(-1),酵母浸粉15 g·L^(-1),KH_2PO_40.5 g·L^(-1),自然pH值。其中氮源为酵母浸粉时对菌SYBS01降解石油的影响最大。添加酵母浸粉后,4 d石油降解率达到85.6%,分别为(NH_4)_2SO_4、NH_4NO_3和尿素的6.7、7.8、2.6倍。进一步分析发现酵母浸粉作为氮源的同时也起到外加营养的作用。在最佳条件下,0.5~5 g·L^(-1)的石油的降解均符合一级动力学模型,且石油浓度为3 g·L^(-1)和4 g·L^(-1)的石油降解半衰期仅为26 h。A petroleum degrading bacterial strain SYBS01 was isolated from oil-contaminated soil,and was identified as Pseudomonas sp. Oil degradation conditions were compared with single- factor and orthogonal experimental design,with the results showing that the optimum conditions were 35 ℃,170 r · min-1,yeast extract15 g·L-1,KH2PO40. 5 g·L-1,and a natural pH value. Among these,the yeast extract had the greatest influence on the oil-degradation rate. The oil-degradation rate of SYBS01 increased to 85. 6% four days after yeast extract had been added to the system,and was 6. 7,7. 8,and 2. 6 times higher than were（ NH4）2SO4,NH4NO3,and CON2H4,respectively. It was shown that yeast extract not only acted as a source of nitrogen,but also provided extra nutrition. A first-order kinetic model could be fitted to the oil degradation for the entire concentration range in optimum conditions. Furthermore,the half-life was found to be 26 h for oil 3 to 4 g·L-1.

关 键 词：石油降解菌 假单胞菌 外加营养 降解动力学 酵母浸粉 

分 类 号：X172[环境科学与工程—环境科学]