辛基酚聚氧乙烯醚高效降解混合菌L9的固定化及其条件优化  

Immobilization of mixed bacteria L9's degrading octylphenol ethoxylates and its optimization

作　　者：刘文斌 张海涛[1] 杨海君[1] 贺强礼[1] 彭晓霞[1] 

机构地区：[1]湖南农业大学植物保护学院,长沙410128

出　　处：《环境工程学报》2016年第10期6056-6064,共9页Chinese Journal of Environmental Engineering

基　　金：湖南省自然科学基金资助项目(2015JJ2079);长沙市科技计划资助项目(K1403022-31)

摘　　要：为彻底高效解决辛基酚聚氧乙烯醚的环境危害,以海藻酸钠和聚乙烯醇为载体,以辛基酚聚氧乙烯醚高效降解混合菌L9为材料,采用海藻酸钠、聚乙烯醇和聚乙烯醇+海藻酸钠制备固定化混合菌L9,研究了各固定化混合菌L9颗粒的机械稳定性与化学稳定性,结果表明,利用聚乙烯醇+海藻酸钠制得的固定化混合菌L9颗粒具有较高的机械和化学稳定性。利用正交实验对聚乙烯醇+海藻酸钠固定化条件进行优化,得到聚乙烯醇浓度8.0%,海藻酸钠浓度1.0%,交联剂2%氯化钙的饱和硼酸溶液,包埋菌量与包埋剂的体积比值2∶1,交联时间24 h为聚乙烯醇+海藻酸钠固定化混合菌L9的最佳条件。考察不同环境条件(温度、pH值和底物浓度)对固定化混合菌L9与游离混合菌L9降解辛基酚聚氧乙烯醚的影响,结果显示,固定化混合菌L9对各环境因素(温度、pH值和底物浓度)的耐受范围都比游离混合菌L9宽,在实际环境应用中更能保持稳定的生物活性,研究结果对解决当前制革、洗涤、农药等行业产生的辛基酚聚氧乙烯醚的环境污染问题具有指导作用。This study examined the elimination of environmental hazards imposed by octylphenyl polyethoxylates（ OPn EO）. The experiment used sodium alginate（ SA） and polyvinyl alcohol（ PVA） as carriers,mixed bacteria L9 for degrading OPn EO effectively as the material,and adopted three different ways,namely SA,PVA,and PVA ＋ SA,to immobilize the mixed bacteria L9 and study the mechanical and chemical stability of three kinds of L9 particles. The results showed that the mixed bacteria L9 particles immobilized by the combination of PVA and SA displayed the highest mechanical and chemical stability. Through Orthogonal Experiments,the optimal PVA ＋ SA immobilization condition was obtained： the calcium chloride saturated boric acid solution with a mixture of 8. 0% polyvinyl alcohol（ PVA）,1. 0% sodium alginate（ SA）,and 2. 0% crosslinking agent,with the volume of embedded bacteria and embedding agent at the ratio of 2 ∶ 1 and the crosslinking time being 24 hours.The analysis of the degradation of OPn EO by the mixed bacteria L9 in both immobilized and free forms,and controlled by different environmental conditions（ temperature,pH,and substrate concentration）,showed that the immobilized mixed bacteria L9 had a wider tolerance range for the aforementioned environmental conditions,compared with those of the free mixed bacteria L9. Thus,our proposed method was found to maintain relatively stable biological activity during actual application and efficiently address the environmental pollution caused by OPn EO,which is used in tanning,detergent,pesticides,and other industrial uses.

关 键 词：固定化 辛基酚聚氧乙烯醚(OPnEO) 混合菌群 生物降解 

分 类 号：X172[环境科学与工程—环境科学]