哺乳动物STE20相关激酶1基因在肝癌组织的表达及其与乙型肝炎病毒的关系  

Expression of mammalian STE20-like 1 gene in hepatocellular carcinoma and its relationship with hepatitis B virus

作　　者：曹军[1] 江平[1] 王波涌[1] 

机构地区：[1]武汉大学中南医院普外科,430071

出　　处：《中华实验外科杂志》2016年第10期2296-2298,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 探讨哺乳动物STE20相关激酶1（MST1）基因在肝癌中的表达水平及与乙型肝炎病毒（HBV）感染及乙型肝炎病毒X蛋白（HBx）的关系.方法 收集临床肝癌组织标本及培养肝癌细胞株,采用Western blot和实时定量聚合酶链反应（Real-time PCR）方法检测MST1的蛋白与mRNA水平及与HBV及HBx的关系.提取感染HBV的细胞检测MST1启动子区甲基化,加入甲基化抑制剂后MST1的变化.结果 32对组织中4对未检测出MST1的表达,其余28对癌组织的MST1 mRNA水平和蛋白水平均明显低于癌旁的表达（P＜ 0.05）.且HBV感染时MST1表达（HepG2 mRNA相对值为13％,HepG2.215 mRNA相对值为7％）明显低于对照组（P＜0.05）.HBV感染细胞后MST1启动子区发生甲基化率为39％,非HBV感染细胞甲基化率为17％,且加入甲基化抑制剂后MST1随抑制剂浓度的升高而增加（P＜0.05）.结论 HBV可能通过甲基化MST1启动子区抑制MST1基因的表达从而进一步导致肝癌发生发展.Objective To explore the relationship between gene mammalian STE20-like 1（MST1） expression and hepatitis B virus （HBV） infection and the hepatitis B virus x-protein （HBx） in hepatocellular carcinoma.Methods Collection of clinical specimens and cultured Hepatic carcinoma cell lines,By Western blotting and real-time quantitative polymerase chain reaction （Real-time PCR） method for detection of protein and mRNA levels and MST1 relationship with HBV and HBx.Extract genome of infection with HBV,Detect methylation of the promoter region.We tested the luciferase activity of MST1 promoter after adding different concentrations of methyl enzyme inhibitors 5-Aza-2＆#39;-deoxycytidine （5-Aza-CdR）.Results MST1 was not deteded in 4 pairs liver tissue.The mRNA and protein espression levels were significantly lower than that of the adjacent tissues in 28 pairs liver tissue （P 〈 0.05）.The expression of MST1 in HBV infection was significantly lower than that of the control group （P 〈 0.05）.The rate of methylation of MST1 promoter region was 39% after HBV infection,the rate of methylation of non HBV infected cells was 17%.And MST1 increases with the inhibitor concentration increasesafter adding 5-Aza-CdR （P 〈 0.05）.Conclusion Expression level of MST1 promoter in the cells containing HBV genome plasmid significantly decreased.

关 键 词：癌  肝细胞 哺乳动物STE20相关激酶1基因 乙型肝炎病毒 甲基化 

分 类 号：R735.7[医药卫生—肿瘤]