96序列相似的家庭成员B在凋亡素特异性诱导HepG2细胞凋亡中的作用  

Function research of family with sequence similarity 96, member B in apoptin-induced apoptosis in the HepG2 cell line

作　　者：蔡逊[1] 马丹丹[1] 张智勇[1] 金炜东[1] 张建新[1] 田俊[2] 

机构地区：[1]广州军区武汉总医院普通外科,武汉430070 [2]华中科技大学同济医学院生物化学与分子生物学系,武汉430032

出　　处：《中华实验外科杂志》2016年第10期2317-2319,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金（81350006）;湖北省自然科学基金（2012FFB06805）

摘　　要：目的 探讨96序列相似的家庭成员B（FAM96B）在凋亡素（Apoptin）特异性诱导人肝癌细胞株HepG2细胞凋亡中的作用.方法 将含人FAM96B全长序列的pcDNA3.1-myc-FAM96B和含人Apoptin全长序列的pcDNA3-HA-Apoptin重组质粒共转染到人肝癌细胞株HepG2细胞中,采用激光共聚焦显微镜观察共转染FAM96B与Apoptin对细胞定位的影响,流式细胞仪检测共转染FAM96B与Apoptin对细胞凋亡的影响.结果 激光共聚焦显微镜观察结果显示,Apoptin定位于HepG2细胞核,FAM96B定位于细胞质.过表达FAM96B通过与Apoptin相互作用可致部分Apoptin定位于细胞质.流式细胞仪检测结果显示：共转染Apoptin和FAM96B的HepG2细胞凋亡率显著低于单独转染Apoptin组[（31.76±1.88）％ vs.（44.85±1.85）％,P＜0.05],表明过表达FAM96B通过与Apoptin相互作用降低Apoptin特异性诱导HepG2细胞的凋亡.结论 共转染FAM96B与Apoptin于HepG2细胞中,过表达FAM96B通过与Apoptin相互作用可致部分Apoptin定位于细胞质,从而抑制Apoptin特异性诱导HepG2细胞的凋亡效应.Objective To investigate the function of family with sequence similarity 96,member B （FAM96B） in apoptin-induced apoptosis in a hepatoma carcinoma cell line HepG2.Methods The recombinant plasmid of pcDNA3.1-myc-FAM96B encoding the human FAM96B gene and pcDNA3-HA-apoptin encoding the human apoptin gene were co-transfected into HepG2 cells.Then laser scanning confocal microscopy was used to observe the celluar localization.The HepG2 cells apoptosis induced by FAM96B and apoptin was detected by flow cytometry.Results The observed results by laser scanning confocal microscopy showed that apoptin was located in the nucleus of the HepG2 cells,while FAM96B in the cytoplasm.FAM96B overexpression resulted in partial apoptin retention in the cytoplasm.Moreover,flow cytometry analysis showed that the apoptosis rate of HepG2 cells in apoptin group was decreased from （44.85 ± 1.85） % to （31.76 ± 1.88） % （P 〈 0.05） in apoptin and FAM96B co-transfection group,indicating that FAM96B interacting with apoptin markedly decreased the apoptosis rate of HepG2 cells induced by apoptin.Conclusion The data revealed that when FAM96B and apoptin were co-transfected into HepG2 cells,FAM96B overexpression reulted in partial distribution of apoptin in the cytoplasm,which significantly inhibited the apoptosis rate of the HepG2 cells induced by apoptin.

关 键 词：96序列相似的家庭成员B 凋亡素 HEPG2 脱噬作用 

分 类 号：R73-3[医药卫生—肿瘤]