胃铋镁对胃癌细胞凋亡的诱导作用及机制研究  

Study on functions and mechanisms of Weibimei in inducing gastric cancer cell apoptosis

作　　者：乔巍[1] 邸军[2] 汪小莞[1] 孙佳昕 张琦[1] 李江[3] 李晓萌[1] 

机构地区：[1]东北师范大学生命科学学院分子表观遗传学教育部重点实验室,吉林长春130024 [2]吉林省人民医院病理科,吉林长春130000 [3]吉林大学口腔医院口腔修复科,吉林长春130041

出　　处：《中国生化药物杂志》2016年第7期15-18,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：科技部国际合作项目资助课题(2010DFA31430);国家自然科学基金(30871301;30700827);吉林省科技厅科研基金资助课题(20130521010JH;20150101187JC;20150414007GH);吉林省教育部科研基金资助课题(2015-526;2015-551);高校基本科研业务费专项基金(2412015ZH005;130017507);高校人才引进项目资助课题(B07017);吉林省产业创新专项(2016C047-3)

摘　　要：目的研究胃铋镁对胃癌细胞株SGC-7901增殖和凋亡的作用及可能机制。方法以三联药(三种市售胃炎胃溃疡类药物联合用药)、胃铋镁、胃铋镁中药总组分、胃铋镁化药总组分为实验组,分别作用于胃癌细胞SGC-7901,MTT法检测细胞的增殖状态;Hoechst33342染色法检测细胞的凋亡;流式细胞仪检测细胞周期;Western blot法检测STAT3信号通路相关蛋白的表达。结果 4种药物分别处理细胞后,相比于对照组和三联药组,胃铋镁组和胃铋镁中药总组分组能够显著抑制细胞增殖且具有剂量依赖性;凋亡百分数显著增高(P<0.05);能将细胞周期阻滞于G1期(P<0.05);Western blot结果显示SGC-7901细胞中STAT3、磷酸化STAT3(p-STAT3)、CyclinD1、Bcl-2蛋白表达量显著降低。结论胃铋镁及其中药总组分可以显著抑制胃癌细胞SGC-7901增殖,促进细胞凋亡,阻滞细胞周期,其作用机制可能与抑制STAT3信号通路相关蛋白p-STAT3、CyclinD1、Bcl-2表达有关。Objective To investigate the proliferative and apoptotic effects of Weibimei on gastric cancer cell and the possible mechanisms.Methods Gastric cancer SGC-7901 cell were cultured with four drugs： Sanlianyao（three kinds of drugs for the treatment of gastric ulcer）,Weibimei,Chinese medicine of Weibimei,and Chemical drugs of Weibimei as the experimental groups. The proliferation of SGC-7901 cell was determined by MTT assays. The cell apoptosis was evaluated by Hoechst33342 staining. The effect on SGC-7901 cell cycle was measured by flow cytometry. The protein expression related to STAT3 signaling pathway in SGC-7901 cell was examined by Western blot. Results After SGC-7901 cells were treated line with each four drug for 24 h,Weibimei or its Chinese medicine portion could obviously inhibit the proliferation of SGC-7901 cells in a dose-dependent manner and increase the percentage of apoptosis rate significantly（P〈 0. 05）. In addition,the cell cycle of SGC-7901 arrested at G1 phase with the general or Chinese medicine portion of Weibimei supplement. Furthermore,Weibimei and its Chinese medicine portion down-regulated the expression of STAT3,phosphorylated STAT3（p-STAT3）,CyclinD1,and Bcl-2 in SGC-7901 cells. Conclusion Weibimei,and its Chinese medicine portion exerted potent anticancer activity by inhibiting cell proliferation,inducing apoptosis and arresting the cell cycle in gastric cancer SCG-7901 cells,and this function was associated with STAT3 signaling inhibition.

关 键 词：胃铋镁 胃癌 STAT3信号通路 

分 类 号：R735.2[医药卫生—肿瘤]