莱菔硫烷抑制宫颈癌细胞系HeLa细胞增殖的机制研究  

Study on the inhibitory effects and mechanism of Sulforaphane on human cervical cancer Hela cell

作　　者：杨晓明[1] 李彩红[1] 孙冬霞[2] 窦海燕[1] 

机构地区：[1]邯郸市妇幼保健院妇一科,河北邯郸056001 [2]邯郸市妇幼保健院病理科,河北邯郸056001

出　　处：《中国生化药物杂志》2016年第7期28-31,共4页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的研究莱菔硫烷对宫颈癌细胞系HeLa细胞增殖的影响,并探讨其可能机制。方法将莱菔硫烷作用于人宫颈癌HeLa细胞,采用MTT和Edu比色法检测细胞体外增殖的抑制作用;采用Western blot检测c-Myc的蛋白表达水平,肿瘤球形成实验检测莱菔硫烷对肿瘤干细胞的影响。结果 MTT和Edu比色法显示,莱菔硫烷能抑制宫颈癌Hela细胞的体外增殖,Western blot结果表明,莱菔硫烷可以下调c-Myc蛋白的表达从而抑制增殖。结论莱菔硫烷对宫颈癌Hela细胞增殖具有明显的抑制作用;其机制可能与下调c-Myc表达有关。Objective To determine the effect of sulforaphane on human cervical cancer HeLa cells as well as its potential mechanism.Methods The proliferation activity were examined by MTT and Edu assay after treated with sulforaphane. Western blot was used to detect the expression level of c-Myc. Results MTT and Edu assay showed that sulforaphane can inhibit the growth of Hela cells,and the results of Western blot indicated that sulforaphane downregulated the expression of c-Myc protein. Conclusion Sulforaphane can significantly suppress the proliferation of HeLa cells. The mechanism may be associated with reduced expression of c-Myc.

关 键 词：莱菔硫烷 宫颈癌 HELA细胞 增殖 C-MYC 

分 类 号：R737[医药卫生—肿瘤]