体外培养人大隐静脉新内膜形成模型的建立  

Experimental Study on Neointimal Formation in an Organ Culture Model of Human Saphenous Vein

作　　者：穆军升[1] 镡朝晖[1] 朱洪生[1] 郑家豪[1] 许德华[2] 刘新垣[2] 

机构地区：[1]上海第二医科大学附属仁济医院心胸外科,上海200001 [2]中国科学院上海分院生化细胞研究所,上海200031

出　　处：《中国胸心血管外科临床杂志》2002年第3期188-191,共4页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

基　　金：卫生部科学研究基金项目 ( 98-2 -2 95 )~~

摘　　要：目的　为了更好地研究静脉再狭窄的机制及预防治疗 ,建立一种人的大隐静脉体外培养模型 ,并对新内膜进行初步研究。　方法　取 6例冠状动脉旁路移植手术患者大隐静脉 ,体外培养 14天 ,常规病理学染色 ,图象分析 ;通过α-平滑肌细胞骨架 (α- actin)免疫组织化学染色方法检测内膜增生细胞 ,末端脱氧核苷酸转移酶介导的 d UTP缺口末端标记 (TUNEL)方法检测内膜凋亡细胞。　结果　培养的大隐静脉在 14天后有新内膜形成和显著的中膜增厚 ,与正常血管相比差别具有显著性意义 (P<0 .0 1)。新内膜细胞α- actin免疫组织化学染色结果呈阳性细胞 ,较正常血管内膜明显增多。在新内膜中荧光和核边聚分裂数目极少 ,与正常血管相比差别无显著性意义。　结论　在人的大隐静脉体外培养中有新内膜形成和中膜增厚 ,增生的细胞可能是肌成纤维细胞 ,故抑制肌成纤维细胞增生迁移的同时 ,促进凋亡将是静脉移植血管病变潜在的治疗方法。Objective To study the mechanism of restenosis in vein as well as its prevention and treatment, neointimal formation was investigate in an organ culture model with human saphenous vein. Methods Saphenous vein segments were obtained from 6 patients undergoing coronary artery bypass grafting(CABG) and cultured. After 14 days, sample was obtained for Histochemical staining and immunohistochemistry. On the other hand, detection of apoptotic cells by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling was carried out. Results Cultured saphenous vein segments developed neointimal formation and marked thickening of the media within 14 days. There was significant difference between normal saphenous veins and cultured saphenous veins( P< 0.01); neointimal cells were positive for α-smooth muscle-actin (α-actin). There was no significant difference between normal saphenous veins and cultured saphenous veins through detection of apoptotic cells. Conclusion These results suggest that cell growth and migration contribute to neointimal formation and medial thickening in cultured human saphenous vein. These neointimal cells are probably myofibroblasts. Inducing apoptosis and inhibiting proliferation of myofibroblasts may have potential therapeutic implications in the treatment for vein graft disease.

关 键 词：冠状动脉旁路移植术 器官培养 大隐静脉 新内膜形成 α-平肖肌细胞骨架 凋亡 血管再狭窄 

分 类 号：R-331[医药卫生]