金匮肾气丸对单侧输尿管梗阻大鼠肾组织p-STAT3蛋白的影响  

Effect of Jingui Shenqi Pill on the Expression of p-STAT3 in Unilateral Ureteral Obstruction Rats

作　　者：刘春燕[1] 朱伟[1] 唐群[1] 梅文娟 陆苗苗[3] 丁志高[1] 

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]南昌大学第一附属医院,江西南昌330006 [3]辽宁医学院附属第一医院,辽宁锦州121001

出　　处：《湖南中医药大学学报》2016年第10期22-26,共5页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金青年项目(81503442);国家自然科学基金青年项目(81603470);湖南省教育厅科研项目(11C0953)

摘　　要：目的探讨金匮肾气丸对单侧输尿管梗阻(unilateral ureteral obstruction,UUO)大鼠肾间质纤维化的影响及对p-STAT3蛋白表达的影响。方法雄性SD大鼠30只,随机分为假手术组、UUO模型组、金匮肾气丸低剂量组、金匮肾气丸高剂量组、阳性对照药依那普利组,UUO术后第1天开始灌胃,第14天处死大鼠,取梗阻肾组织,HE和Masson染色观察肾组织病理改变,免疫组织化学检测I型和III型胶原在肾组织的蛋白表达,Real-time PCR法检测I型和III型胶原在肾组织的m RNA水平,Western印迹检测磷酸化的信号转导与转录激活因子3(signal transducer and activator of transcription 3,STAT3)在肾组织的表达。结果与UUO模型组比较,金匮肾气丸治疗后,肾间质损伤指数降低,间质胶原阳性面积减少,I型和III型胶原蛋白及m RNA表达减少,磷酸化STAT3蛋白水平降低(P<0.05)。结论金匮肾气丸可以减轻UUO大鼠梗阻肾组织肾间质损伤,减少间质内胶原沉积,其作用机制可能与抑制STAT3磷酸化有关。Objective To investigate the effect of Jingui Shenqi pill on unilateral ureteral obstruction（UUO） rats with renal interstitial fibrosis and the expression of p-STAT3 protein. Methods 30 male SD rats were randomly divided into sham operation group, UUO model group, low dose of Jingui Shenqi pill group, high dose of Jinguishenqi pill group, enalapril treatment group as positive control. After intragastric administration on the 14 days, the rats were sacrificed. The obstructive kidney tissues were stained with HE and Masson to observe the pathologic changes. The protein or m RNA expression of type I and III collagen in renal tissue was detected by immunohistochemistry staining or Real-time PCR. The phosphorylation of signal transducer and activator of transcription 3（STAT3） in renal tissue was detected by Western blot. Results Compared with UUO model group, after treatment with Jingui Shenqi pill, the renal interstitial damage index and collagen positive area were decreased, the expression of type I and type III collagen protein and m RNA were also decreased and the phosphorylation of STAT3 protein was inhibited（P〈0.05）. Conclusion Jingui Shenqi pill can obviously improve renal interstitial injury and reduce interstitial collagen deposition in UUO rats which may be related to the inhibition of STAT3 phosphorylation

关 键 词：金匮肾气丸 肾纤维化 单侧输尿管梗阻 大鼠 p-STAT3蛋白 熟地黄 山茱萸 山药 

分 类 号：B277.5[哲学宗教—中国哲学] B285.5