降香水提物和挥发油对心肌缺血/再灌注损伤大鼠预防作用的代谢组学研究  

Metabolomic study on preventive effect of Aqueous extract and Volatile oil of Dalbergia Odorifera on myocardial ischemia/reperfusion injury in rats

作　　者：牟菲[1] 段佳林[1] 边海旭 蔺瑞[1] 尚沛津 朱志会[1] 文爱东[1] 奚苗苗[1] 

机构地区：[1]第四军医大学西京医院药剂科,陕西西安710032

出　　处：《中国药理学通报》2016年第10期1377-1382,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金面上项目(No 81470174)

摘　　要：目的研究心肌缺血/再灌注(MI/R)损伤大鼠经降香水提物(DOA)和降香挥发油(DOO)治疗后血清代谢物的变化,探讨MI/R损伤发病及药物作用机制。方法采集假手术组、MI/R模型组、DOA给药组和DOO给药组大鼠的血清样本,应用气相飞行时间质谱联用系统(GC-TOF-MS)对样本进行代谢图谱分析,将数据预处理后导入SIMCA 14.1软件进行多元统计学分析。结果经主成分分析(PCA)、偏最小二乘法分析(PLS-DA)及正交偏最小二乘法分析(OPLSDA),模型组与假手术组明显分离,给药组与模型组分离,并向假手术组靠近,在代谢组学的角度证明了DOA和DOO对MI/R损伤大鼠的治疗作用,实验共分析鉴别出13个内源性生物标记物,分别与糖代谢、脂代谢和氨基酸代谢通路相关。结论 DOA和DOO可以对MI/R损伤大鼠起到很好的保护作用,推测其可能是通过调节糖代谢、脂代谢和氨基酸代谢通路而发挥作用。Aim To investigate the changes of serum metabolism after the treatment of DOA and DOO on myocardial ischemia reperfusion （ MI/R ） injury in rats, and to explore the pathogenesis of MI/R injury and drug action mechanism. Method The serum samples of Sham group, Model group, DOA group and DOO group of rats were acquired, gas phase time of flight mass spectrometry （ GC-TOF-MS） was applied to analyze the metabolic profiles of the samples. After da-ta preprocessing, they were processed into SIMCA 14. 1 software for multivariate statistical analysis. Results By principal components analysis （ PCA ） , partial least squares analysis （ PLS-DA） and orthogonal partial least squares analysis （ OPLS-DA ） , the Model group and Sham group were obviously separated, the drug in-tervention group and Model group were separated and close to Sham group. The therapeutic effect of DOO and DOA on MI/R injury in rats was proved. The ex-perimental results identified 13 endogenous biomark-ers, which were related to the glucose metabolism,lipid metabolism and amino acid metabolism pathway. Con-clusion DOA and DOO may protect the MI/R injured rats by regulating the glucose metabolism, lipid metab-olism and amino acid metabolism pathway.

关 键 词：代谢组学 气相飞行时间质谱 心肌缺血/再灌注损伤 降香水提物 降香挥发油 能量代谢 

分 类 号：R-332[医药卫生] R284.1