基于荧光定量PCR评测热性中药成分对TRPV1通道功能的影响  

Evaluation of regulational function of ingredients from hot herbs on TRPV1 channel based on 7900 PCR instrument

作　　者：周海玉[1] 戴丽[1] 王德凤[1] 戴逸飞[1] 周炜炜[1] 孟晶[1] 隋峰[1] 霍海如[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国药理学通报》2016年第10期1395-1398,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金面上项目(No 81274112;81373986;81473372);国家自然科学基金青年项目(No 81403125;81403322);北京市自然科学基金项目(No 7152106)

摘　　要：目的深入研究热性中药的生物学功能和分子机制。方法该研究选择了临床常用的热性中药的主要活性成分,基于新型7900PCR仪尝试搭建背根神经节(DRG)神经元TRPV1通道温觉感知功能的检测评价系统,并对热性中药成分的TRPV1通道功能的调节作用进行了研究。结果TRPV1通道能够被温度升高所激活,且该激活过程可被TRPV1特异性阻断剂辣椒平抑制,所选大部分热性中药成分均具有上调TRPV1通道功能的作用。结论这些结果提示基于PCR仪建立的TRPV1通道功能检测体系适于药物调节TRPV1通道功能的研究;热性中药表征的热性属性可能与其所含的活性成分能够提高TRPV1通道感知温度的活性,从而增加机体的能量代谢有关。Aim To further study the molecular mecha-nism of the herbs with hot nature on the regulational action on TRPV1 channel based on the 7900 Real-time PCR instrument. Methods 7900 PCR instrument was applied to detect the intracellular flurescence of TRPV1 channel in the dorsal root ganglions （ DRG ） neurons and the effect on the TRPV1 ’ s thermo-sensational functions of the selected 11 ingredients from hot herbs was explored. Results TRPV1 channels could be ac-tivated by gradually elevated temperature; the activa-tion process could be blocked by the TRPV1 specific blocking agent capsaizepine. Most of the ingredients from hot-nature herbs had the potential to up-regualate TRPV1 channel function. Conclusions The estab-lished TRPV1 channel detection system based on PCR instrument is suitable for the analysis of regulational functions of drugs on the heat-activated TRPV1 chan-nel;the functions of hot herbs may be related to the up-regualtional effects of its active ingredients on the TRPV1 channel which will further up-regulate energy metabolism.

关 键 词：热性中药成分 背根神经节 TRPV1通道 7900PCR仪 温觉感受功能 能量代谢 

分 类 号：R-332[医药卫生] R282.71