3种不同血瘀证动物模型的建立与评价  

The establishment and evaluation of three different kinds of animal model of blood stasis syndrome

作　　者：梁耀月 李佳佳[1] 董世芬[1] 张荣[1] 袁悦莹 孙建宁[1] 

机构地区：[1]北京中医药大学中药学院中药药理系,北京100102

出　　处：《中国药理学通报》2016年第10期1468-1473,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81503287,81430094);教育部博士点基金项目(No 20130013120002)

摘　　要：目的建立痰浊血瘀、气滞血瘀、痰浊+气滞血瘀大鼠模型,研究不同血瘀状态动物模型的特征。方法选用SD大鼠,随机分为正常对照组、高脂饲料组、慢性刺激组(chronic unpredictable mild stress,CUMS)、高脂饲料+慢性刺激组,分别采用相应因素干预6周。于2、4、6周动态监测动物体重、旷场行为、血脂和血清皮质酮指标;实验终点(6周)采用小动物超声和左心室插管法检测心功能,采用黏度仪、红细胞变形/聚集测试仪检测血液流变性相关指标。结果与正常对照组大鼠相比,采用高脂饲料、慢性不可预知性刺激后均可引起血瘀状态,对动物的行为学、血脂、心脏功能、血液黏度造成了不同程度的影响,当两因素叠加时,血瘀相关指标变化最明显,表现为:与正常对照组比较,体重明显降低(P<0.01),2周时水平运动、垂直运动、运动时间均减少(P<0.01或P<0.05),2周、4周时,血清皮质酮上升(P<0.01或P<0.05),4周、6周时TG明显上升(P<0.01)。6周时,血流速度减慢(P<0.01),收缩末期左室前壁、后壁厚度均增厚(P<0.01或P<0.05),舒张期左心室指数上升(P<0.01),左心室最大收缩速率降低(P<0.05),全血黏度上升(P<0.01)。结论高脂饲料喂养和慢性不可预知性刺激均可形成血瘀状态,但是具有不同的特点。当两因素叠加时,动物可明显出现行为学、血液黏度、心脏功能、血脂等指标的异常,可为血瘀证以及相关药物的研究提供依据。Aim To establish phlegm and blood stasis, qi-stag-nation and blood stasis, phlegm turbid＋qi-stagnation and blood stasis model in rats and to study the characteristics of animal models with different blood stasis. Methods SD rats were ran-domly divided into normal group, high fat diet group, chronic unpredictable mild stress group （ CUMS ） and high fat diet ＋chronic unpredictable mild stress group. Different states of blood stasis rat models were established by corresponding factors for 6 weeks. Indexes of weight, open field behavior, serum lipids and corticosterone were monitored dynamically at the 2 nd, 4 th, 6 th weeks. At the end of the experiment（6th week）, the heart func-tion was detected by small animal ultrasound and the left ventric-ular intubation. The blood rheology indexes were detected by the viscosity tester and red blood cell deformation/aggregation test instrument. Results Compared with the normal group, blood stasis could be induced by high fat diet and chronic unpredicta-ble mild stress, introducing the influence of different degree on animal behavior, blood lipids, heart function and blood viscosi-ty. When the two factors were superimposed, the changes of the indexes about blood stasis were the most significant. Perform-ance as： compared with normal control group, a significant re-duction was observed in body weight （ P 〈 0. 01 ）; horizontal movement, vertical movement and movement time were reduced （P〈0. 01 or P〈0. 05） at the 2 nd week;at the 2 nd and 4 th week, serum corticosterone was increased （ P 〈0. 01 or P 〈0. 05） as well as TG at the 4 th and 6 th week （P〈0. 01）; at the 6 th week, velocity of blood was slowed down （ P〈0. 01 ）;left ventricular anterior wall and posterior wall thickness at end-systolic was increased （ P〈0. 01 or P〈0. 05 ）; left ventricular diastolic index was increased （ P〈0. 01 ）; the maximum rate of myocardial contraction was decreased （ P 〈 0. 05 ）; the whole blood viscosity was increased （ P〈0. 01

关 键 词：慢性不可预知刺激 高脂饲料 气滞 痰浊 血瘀证 动物模型 

分 类 号：R-332[医药卫生] R228