人胎盘底蜕膜间充质干细胞纹状体移植治疗帕金森病模型大鼠的实验研究  

Experimental study of hPDB-MSCs striatum transplantation in the treatment of PD on rat models

作　　者：肖振勇[1] 卢国辉[2] 覃军[1] 闫宪磊[1] 陈家康[1] 李学东[1] 

机构地区：[1]广西医科大学第四附属医院暨柳州市工人医院神经外科,柳州545005 [2]南昌大学第一附属医院神经外科,南昌330006

出　　处：《右江医学》2016年第5期485-489,共5页Chinese Youjiang Medical Journal

基　　金：国家自然科学基金(81560220)

摘　　要：目的研究人胎盘底蜕膜间充质干细胞(hPDB-MSCs)纹状体移植对帕金森病(PD)模型大鼠行为学的影响。方法体外传代培养hPDB-MSCs,使用6-OHDA制备大鼠PD模型并随机分为移植组、假手术组、模型组,对移植组大鼠右侧纹状体进行hPDB-MSCs移植,术后1 W、2 W、4 W进行行为学检测。免疫组化检测移植后1 W、2 W及4W各组大鼠损伤侧黑质酪氨酸羟化酶(TH)的表达。结果 hPDB-MSCs移植后2 W、4 W,移植组大鼠阿朴吗啡诱导的平均旋转圈数明显低于假手术组及模型组(P<0.01);免疫组化显示移植组损伤侧黑质部位移植后4 W TH阳性细胞数较移植前明显增加(P<0.01);与假手术组相比,移植术后1 W、2 W、4 W移植组TH阳性细胞明显增多(P<0.01)。结论 hPDB-MSCs移植能够抑制PD模型大鼠多巴胺能神经元丧失,改善PD模型大鼠的行为学症状,具有一定的治疗作用。Objective To investigate the effect of human placental decidua basalis-derived mesenchymal stem cells（hPDB- MSCs） striatum transplantation in the treatment of Parkinson＇s disease（PD） on rat models. Methods hPDB-MSCs were cultured in vitro.PD rat models were prepared by 6-OHDA and divided into transplantation group, sham operation group and model group. HPDB-MSCs transplantation was performed on the right striatum of the transplantation group, and behavioral detection was carried out 1W,2W and 4W after opemtion.Immunohistochemical method was used to detect expression of tyrosine hydroxylase （TH） in w-ounded sides among all the groups 1W,2W and 4W after transplantation. Results 2W and 4W after transplantation,mean rota-tional circle induced by apomorphine（APO） in the transplantation group w-ere low-er than those in the sham operation group and the model group（P 〈0.01）.Immunohistochemistry show-ed that TH positive cell numbers of the substantia nigra in the w-ounded sides 4W after transplantation significantly increased compared wdth those before transplantation in the transplantation group（P 〈0.01）.Com- pared with the sham operation group, TH positive cell numbers 1W,2W and 4W after transplantation in the transplantation in-creased more significantly（P 〈0.01）.Conclusion hPDB-MSCs transplantation can inhibit the loss of dopaminergic neuron, improve behavioral symptoms of PD model rats, it has certain treatment effects.

关 键 词：间充质干细胞 底蜕膜 神经保护 帕金森病 

分 类 号：R742.5[医药卫生—神经病学与精神病学] R457.7[医药卫生—临床医学]