牛磺酸上调基因1在肾癌组织的表达及临床意义  

Expression of taurine up-regulated gene 1 and the clinical significance in renal cell carcinoma

作　　者：全晶[1] 金露[1] 潘翔[1] 桂耀庭[2] 杨尚琪[3] 毛向明[3] 来永庆[3] 

机构地区：[1]安徽医科大学研究生学院,合肥230032 [2]北京大学深圳医院男性生殖实验室 [3]北京大学深圳医院泌尿外科

出　　处：《中国综合临床》2016年第9期776-779,共4页Clinical Medicine of China

基　　金：国家自然科学基金项目（81101922）;深圳市科技研发资金知识创新基础研究项目（JCYJ20150403091443304）

摘　　要：目的：探讨牛磺酸上调基因1（ TUG1）在肾癌组织及其配对癌旁正常组织中的表达，及其与临床病理特征之间的相关性。方法从46例经手术完整切除的肾癌组织及其配对癌旁正常组织中分别提取获得各自的总RNA，经逆转录得到cDNA，使用RTqPCR检测两者TUG1的表达，分析TUG1表达与患者临床病理特征的相关性。结果 TUG1在肾癌组织中的表达明显低于配对的癌旁正常组织（0．533±0．027、1．000±0．298），差异有统计学意义（t＝－3．350，P＜0．01）。46例肾癌组织中TUG1的△CT值经对数转换后最小值为－5．535，最大值3．085，平均值－0．908，以平均值－0．908为分界线，46例肾癌组织中有25例（54．34％）呈表达下调。 TUG1的表达水平与肾癌患者的年龄、性别、肾癌组织类型、TNM分期以及UICC／AJCC临床分期无显著相关性（ P均＞0．05）。结论肾癌组织中TUG1表达下调，提示其可能与肾癌的发生发展相关。Objective To detect the expression level of Taurine upregulated gene 1（ TUG1） in the renal cell carcinoma and paired paracancerous normal tissues,then explore the relationships between the expression level of TUG1 and clinical characteristics.Methods RNA was Extacted from the resected renal cell carcinoma tissues and paired paracancerous normal tissues of 46 patients respectively,by reverse transcription to get cDNA, the expression level of the TUG1 was detected by RTqPCR, the relationship between the expression level of TUG1 and the clinicopathological characteristics was analyzed by statistically software. Results The expression of TUG1 in renal cell carcinoma was obviously lower than that in paired paracancerous normal tissues（0.533±0. 027 vs. 1.000±0.298,t=-3.350,P〈0.01）.The△CT value of Tug1 in 46 cases of renal cell carcinoma after logarithmic transformation,the minimum value was -5.535,maximum was 3.085,average value was -0.908,with the average of -0.908 as a dividing line,46 cases of renal cell carcinoma with 25 cases （54.34%） were down regulated the expression.The expression level of TUG1 of patients with renal carcinoma have no significant correlation with age,sex,type of renal cell carcinoma,TNM staging and UICC/AJCC staging（P〉0.05）.Conclusion The expression of TUG1 in renal cell carcinoma tissues are downregulated,which also suggest that it may be related to the tumorigenesis and development of renal cell carcinoma.

关 键 词：肾癌 肿瘤分期 牛磺酸上调基因1 TAURINE up-regulated gene 1 

分 类 号：R737.11[医药卫生—肿瘤]