沙门菌-斑马鱼感染模型用于Th1/Th2免疫应答的研究  

Th1/Th2 Immune Response in a Salmonella-zebrafish Infection Model

作　　者：徐光美[1] 王俐丹 李嫄渊[1] 吴淑燕[1] 黄瑞[1] 

机构地区：[1]苏州大学基础医学与生物科学学院病原生物学系,江苏苏州215123

出　　处：《现代生物医学进展》2016年第29期5606-5609,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金面上项目(81471572)

摘　　要：目的:通过构建斑马鱼成鱼感染模型,研究鼠伤寒沙门菌感染对机体Th1/Th2免疫应答的影响。方法:用不同剂量细菌口饲感染8月龄的斑马鱼成鱼,绘制3周生存率曲线。观察各剂量下对成鱼的感染情况,并用实时荧光定量聚合酶链式反应检测肝脏Th1、Th2型细胞相关基因和细胞因子的mRNA转录水平,计算Th2/Th1相对表达量比值。结果:用105 CFU感染2周斑马鱼全部存活,第15天开始出现死亡,且在3周后死亡率达到50%;感染后3周解剖发现,肝脏、脾脏和肠道有明显红肿和糜烂;肝脏Th1、Th2型细胞相关基因和细胞因子mRNA转录水平明显向Th2偏移。结论:用105 CFU鼠伤寒沙门菌口饲感染斑马鱼成鱼构建的模型,能反映机体感染和免疫功能变化,可用于研究体内Th1/Th2免疫应答,为进一步研究鼠伤寒沙门菌感染与免疫机制提供了很好的实验工具。Objective： A Salmonella-zebrafish infection model was established to investigate the Th1/Th2 immune response initi- ated by pathogenic bacteria. Methods： 8-month-old zebrafish was infected by oral administration with different CFU. The survival curve was drawn to show the life-or-death situation. The infection of adults＇ viscera was observed with the naked eye after that they are dissect- ed after 3 weeks. The expression levels of Th 1, Th2 cell associated gene and cytokines mRNA in liver were measured by real-time quan- titative PCR and the ratio of corresponding cytokines for Th2/Th1 were also calculated. Results： All of adults were alive within 2 weeks infected by 105 CFU infectious dose, while the death occurred on the 15th day. The death rate was 50 % three weeks later. With serious red, swollen and erosion, the lesions of liver, spleen and intestine were significant, which were gained by means of dissecting the adults. It was discovered that an obvious Th1/Th2 immune response was associated with 105 CFU infectious dose. Under this dose, a Th2 re- sponse swift appeared evidently. Conclusions： It was feasible to establish a Salmonella-zebrafish infection model with 105 CFU oral ad- ministration dose. The model was appropriate for reflecting infection and the immune function in the zebrafish adults, which could be used to investigate Th1/Th2 immune response induced by pathogens. It was an available experimental implement to in-depth study the mechanism of infection and immune induced by Salmonella typhimurium.

关 键 词：鼠伤寒沙门菌 斑马鱼 TH1/TH2免疫应答 

分 类 号：R-33[医药卫生] R378