硫辛酸对卵巢切除小鼠学习记忆的影响  

Effect of Lipoic Acid on Learning and Memory Capacity of Ovariectomized Mice

作　　者：梁琛[1] 李伟[2] 邵晓平 王意雯 唐惠[3] 

机构地区：[1]徐州医学院,江苏徐州221000 [2]徐州医学院附属医院内分泌科,江苏徐州221002 [3]徐州医学院公共卫生学院,江苏徐州221000

出　　处：《现代生物医学进展》2016年第29期5636-5639,共4页Progress in Modern Biomedicine

基　　金：江苏省自然科学基金项目(BK20150218)

摘　　要：目的:探讨硫辛酸(Lipoic acid,LA)对卵巢切除(ovariectomized,OVX)小鼠学习记忆的影响。方法:2月龄雌性昆明小鼠随机分为4组:假手术(Sham)组、OVX组、OVX+LA组、OVX+LA+左旋硝基精氨酸甲酯(L-NAME)组。建立卵巢切除模型并给药,Morris水迷宫检测各组小鼠学习记忆能力。结果:前4天定位巡航实验中,OVX组与Sham组相比,逃避潜伏期明显延长(P<0.05),第5天撤除平台后,OVX组在目的象限游泳距离及时间百分比较Sham组明显缩短(P<0.05);OVX+LA组与OVX组相比,逃避潜伏期明显缩短(P<0.05),撤除平台后,在目的象限游泳距离及时间百分比明显延长(P<0.05);OVX+LA+L-NAME组与Sham组相比,逃避潜伏期明显延长(P<0.05),撤除平台后,在目的象限游泳距离及时间百分比明显缩短(P<0.05)。结论:OVX损伤小鼠的学习记忆,LA可改善这种损伤,内皮型一氧化氮合酶(e NOS)抑制剂L-NAME可削弱LA的这种保护作用。Objective： To investigate the influence of Lipoic acid （LA） on learning and memory abilites of Ovariectomize （OVX） mice. Methods： 2-month-old female Kunming mice （22-25 g） mice were assigned randomly to a sham operated （Sham） group, ovariec- tomized （OVX） group, ovariectomized＋Lipoic acid （LA） group, and ovariectomized＋ LA＋L-NAME group. Morris water maze was used to test the learning and memory abilities of mice. Results： Compared with the Sham group, in the OVX group, the average escape latency detected by hidden platform trial were higher （P〈0.05）, the proportion of time and swim distance the mice spent searching for the plat- form in the target quadrant were lower （P〈0.05）; as compared with the OVX group, LA shortened the escape latency （P〈0.05）, improve the proportion of time and swim distance（P〈0.05）; compared with the Sham group, in the OVX＋LA＋L-NAME group, the average escape latency were higher （P〈0.05）, the proportion of time and swim distance the mice spent searching for the platform were lower （P〈0.05）. Conclusions： LA could improve the decreased of spatial learning and memory ability of mice which was induced by OVX. After an injec- tion of L-NAME, the above effects of LA were attenuated.

关 键 词：卵巢切除 硫辛酸 学习记忆 ENOS 

分 类 号：R-33[医药卫生] R711.51