超声内镜引导下细针穿刺活检联合基因检测对胰腺癌的诊断价值  

Diagnostic Value of Endoscopic Ultrasonography Guided Fine Needle Aspiration Biopsy Combined with Gene Detection for Pancreatic Cancer

作　　者：孙瑞[1] 蔡迎彬[2] 李丹萍[2] 张琪琪[2] 王硕[2] 侯慧[1] 

机构地区：[1]新疆医科大学第五附属医院超声诊断科,新疆乌鲁木齐830011 [2]新疆医科大学第五附属医院消化内科,新疆乌鲁木齐830011

出　　处：《现代生物医学进展》2016年第30期5921-5922,5842,共3页Progress in Modern Biomedicine

基　　金：2015年新疆自治区卫生计生委青年科技人才专项科研项目(2015Y27)

摘　　要：目的:研究超声内镜引导下细针穿刺活检(EUS-FNA)联合K-ras基因检测对胰腺癌的诊断价值,为临床诊疗提供依据。方法:选取2013年11月到2015年11月我院收治的胰腺占位病变患者90例,患者入院次日行EUS-FNA,检测患者血清及活检物中K-ras基因阳性率,比较EUS-FNA单独与EUS-FNA联合K-ras基因检测对胰腺癌诊断的准确率与敏感性。结果:90例胰腺占位病变者中,经病理证实胰腺癌56例,EUS-FNA单独与EUS-FNA联合K-ras基因分别检出胰腺癌50例、53例,准确率分别为89.29%、94.64%,敏感性分别为92.59%、98.15%,两组比较差异均有统计学意义(P<0.05)。胰腺癌患者活检物中K-ras阳性率为83.93%,明显高于血清中的41.07%(P<0.05)。结论:EUS-FNA联合K-ras基因检测可提高对胰腺癌诊断的准确率与敏感性。Objective： To study the diagnostic value of endoscopic ultrasonography guided fine needle aspiration biopsy （EUS-FNA）combined with K-ras gene detection for pancreatic cancer, so as to provide basis for clinical diagnosis and treatment. Methods： 90 cases of patients with pancreatic occupying lesions were selected treated in our hospital from November 2013 to November 2015, who were underwent EUS-FNA the next day after admission, then detected the positive rates of K-ras gene in serum and biopsy, and compared the accuracy rate and sensitivity between EUS-FNA and EUS-FNA combined with K-ras gene detection on the diagnosis of pancreatic cancer. Results： 56 patients were confirmed with pancreatic cancer by pathology detection among 90 patients with pancreatic occupying lesions, EUS-FNA and EUS-FNA combined with K-ras gene detection found 50 cases and 53 cases respectively, and the accuracy rate were 89.29% vs 94.64%, sensitivity were 92.59% vs 98.15%, the differences were statistically significant（P〈0.05）. The positive rate of K-ras in biopsy was 83.93%, which was significantly higher than 41.07% in serum of patients with pancreatic cancer （P〈0.05）. Conclusion： EUS-FNA combined K-ras gene detection could increase the accuracy rate and sensitivity for the diagnosis of pancreatic cancer.

关 键 词：超声内镜 细针穿刺活检 K-RAS 胰腺癌 诊断 

分 类 号：R735.9[医药卫生—肿瘤]