宫颈液基细胞学样本中DAPK基因甲基化的检测及临床意义  

Detection of DNA methylation of DAPK gene in cervical liquid-based cytology specimens and its clinical significance

作　　者：谢玲红 顾栋桦[2] 

机构地区：[1]浙江省湖州市计划生育宣传技术指导站,313000 [2]浙江省湖州市中心医院

出　　处：《中国计划生育学杂志》2016年第10期673-676,共4页Chinese Journal of Family Planning

摘　　要：目的：探讨在液基细胞学样本中检测宫颈鳞状细胞癌及其癌前病变的DAPK基因的甲基化临床意义。方法：收集2014年6月-2015年6月在本单位就诊或体检妇女的宫颈液基细胞学检查样本231例,在满足细胞学诊断后,用甲基化特异性聚合酶链反应检测样本中DAPK基因的甲基化状态,同时做宫颈组织病理学检查。结果：总体样本中宫颈液基细胞学DAPK基因的甲基化率为16.9%（39/231）,NILM、ASC-US、LISL、HISL、SCC各组中,DAPK基因的甲基化率分别为3.8%（2/53）、3.9%（2/52）、13.8%（8/58）、37.1%（23/62）、66.7%（4/6）,HISL组和SCC组基因甲基化率高于NILM、ASC-US和LISL组。根据宫颈组织病理学诊断结果,宫颈炎、宫颈湿疣、宫颈上皮内瘤变（CIN）、鳞状细胞癌（SCC）各组中,DAPK基因的甲基化率分别为3.6%（2/55）、4.0%（2/50）、24.8%（28/113）、53.9%（7/13）,SCC组和CIN组DAPK基因甲基化率高于宫颈炎组和宫颈湿疣组;SCC组基因甲基化率高于CIN组。CINⅢ组DAPK基因甲基化率高于CINⅠ及CINⅡ组,而CINⅠ及CINⅡ组间无统计学差异。结论：宫颈液基细胞学样本中,SCC和HISL的DAPK基因的甲基化率相对较高,临床上可以利用少量液基细胞学检测样本,作为诊断宫颈癌及癌前病变的辅助诊断指标。Objective： To explore the clinical significance of DAPK gene methylated in detecting squamous cell carcinomas and squamous intraepithelial lesions of cervical liquid-based cytology specimens. Method： From June 2014 to June 2015, 231 liquid based cervical cytology samples from women who had visited hospital or had received physical exami- nation were collected. After Cytological diagnosis, the residual specimens were used to examine methylation of DAPK gene by methylation-specific polymerase chain reaction approach. Result.. The total rate of DAPK gene methylation was 16.9% （39/231）. Rate of DAPK gene methylation in NILM group, ASC - US group, LISL group, HISL group, or SCC group was 3.8% （2/53）, 3.9% （2/52）, 13.8% （8/58）, 37.1% （23/62）, 66.7% （4/6）, respectively. The rate of DAPK gene methylation in SCC or HISL group was significantly higher than that in NILM, ASC-US or LISL group. According to cervical biopsy specimens examination, the rate of DAPK gene methylation in chronic cervicitis group, cervical condylomata group, CIN or SCC group was 3.64%（2/55）, 4.00%（2/50）, 24.78% （28/113）, 53.85%（7/13）, respectively. The rate of DAPK gene methylation in SCC group or CIN group was significantly higher than that in chronic cervieitis or cervical condylomata group, and the rate of DNA methylation in SCC group was higher than that in CIN group. For CIN samples, The rate of DAPK gene methylation was significantly in CINN group was higher than that in CIN Ⅰ group and CIN Ⅱ group, but there was no significant difference between CIN Ⅰ group and CIN Ⅱ group. Conclusion： In liquid based cervical cytology samples, The DNA methylation of DAPK gene in HSIL group or SCC group is higher, so detection of DAPK gene methylation can be as an auxiliary diagnostic indicator to cervical cancer and precancerous lesions.

关 键 词：甲基化 DAPK基因 宫颈液基细胞 

分 类 号：R737.33[医药卫生—肿瘤]