腹部枪弹伤合并海水浸泡猪模型的建立  

Establishment of the swine model of abdominal war injury coupled with seawater immersion

作　　者：林孝文[1,2] 祁亚峰[2] 张再重[2] 黄盛[2] 夏印[2] 王瑜[2] 王烈[2] 

机构地区：[1]福建中医药大学附属人民医院 南京军区福州总医院普通外科,福州350000 [2] 南京军区福州总医院普通外科

出　　处：《中华航海医学与高气压医学杂志》2016年第4期273-277,共5页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：南京军区医学科技创新项目（13ZX29）,南京军区医学科技创新项目（14ZX25）

摘　　要：目的：建立稳定性、可重复性强的枪弹伤合并海水浸泡动物模型。方法20只巴马小型猪按数字表法随机分成海水组和地面组，每组10只。海水组使用国产81-1式突击步枪距离10 m实弹射击半漂浮于海中的巴马小型猪，致其左下腹反麦氏点受伤，海水中浸泡60 min后打捞出水；地面组在海岸边陆地上以同样方式致伤，不进行海水浸泡。以打捞出水时间为基点（地面组致伤后等待60 min），监测120 min内2组体温、心率、呼吸、血压指标变化，统计死亡率。打捞后120 min行剖腹探查，统计2组腹腔脏器损伤情况，取肝、肺、肠标本，光镜下观察病理变化。结果打捞后120 min内，地面组与海水组呼吸频率随时间呈升高趋势，2组同时间点比较差异均有统计学意义（P＜0．05）；海水组心率先增高后降低，地面组心率未见明显变化，2组心率总体比较差异有统计学意义（P＜0．05）；海水组体温随时间延长而升高，地面组体温未见明显变化，2组同时间点比较差异有统计学意义（P＜0．05）；海水组血压呈先升高后降低的变化，而地面组血压未见明显变化，2组收缩压，舒张压比较差异均无统计学意义（P＞0．05）。2组脏器损伤及肠穿孔数差异无统计学意义（P＞0．05）；损伤肠管的口径统计，2组出、入口径均差异有统计学意义（P＜0．05）；海水组与地面组死亡率分别为30％和10％，2组比较差异无统计学意义（P＞0．05）。海水组各脏器病理示炎症、水肿程度均较地面组严重。结论腹部枪弹伤合并海水浸泡猪模型高度模拟了濒海战场腹部枪弹伤情景，充分融入海水浸泡与枪弹致伤因素，适用于濒海实地腹部战创伤救治的相关研究。Objective To establish the stable and reproducible swine model of abdominal war injury coupled with seawater immersion.Methods Twenty Obama miniature swine were randomly divided into the seawater group and the ground group,each consisting of 10 swine.The animals in the seawater group were injured by the home-made type 81 -1 rifle shot at the McIntosh point of the left abdomen from a distance of 10 m,when they were half floated in the seawater.They were salvaged following 60 min of seawater immersion. The animals in the ground group were injured in the same way,but without seawater immersion.With the salvage time out of water as the reference time point （60-min seawater immersion for the animals of the seawater group and also the same length of time for the animals of the ground group）,the body temperature,heart rate, respiration and blood pressure indexes in 120 min were detected for the animals of the 2 groups,and the death rate of the 2 groups was also statistically analyzed.One hundred and twenty minutes after salvage from the water,exploratory laparotomy was performed to detect the injuries of the abdominal organs in animals of the 2 groups.Then,liver,lung and intestine samples were collected through laparotomy,and pathological changes＆amp;nbsp;were observed under light microscopy.Results One hundred and twenty minutes after salvage,respiratory frequency of the 2 groups all increased with the extension of time,and statistical significance could be noted when comparisons were made between the same time points（P〈0.05 ）.Heart rate of the seawater group was first elevated,and then was decreased,while no significant changes in the ground group was noticed,and no statistical significance could be seen in heart rate when comparisons were made between the 2 groups （P〈0.05）.Body temperature of the seawater group all increased with the extension of time,while no significant changes could be seen in the ground group,and statistical significance could be found in body temperature when comparisons were m

关 键 词：枪弹伤 濒海 模型建立 

分 类 号：R-332[医药卫生] R82