miR-130a在肝癌组织中的表达及对肝癌细胞迁移侵袭的影响  

Expression of miR-130a and its effect on migration and invasion of hepatocellular carcinoma

作　　者：张雯雯[1] 王宏光[1] 陈明易[1] 张文智[1] 何蕾[1] 史宪杰[1] 

机构地区：[1]解放军总医院肝胆外科,北京100853

出　　处：《解放军医学院学报》2016年第10期1090-1094,共5页Academic Journal of Chinese PLA Medical School

摘　　要：目的研究miR-130a在肝癌组织中的表达、对肝癌迁移侵袭的影响和这种影响与上皮间质转化(epithelialmesenchymal transition,EMT)途径的关系。方法通过q RT-PCR检测肝癌组织标本和高转移性肝癌细胞株中miR-130a的表达,验证miR-130a在高转移性肝癌细胞株(MHCC97-H、HCCLM3)中的表达对肝癌细胞迁移侵袭能力的影响,并研究这种影响与EMT相关分子(MMP-9和MMP-2)的关系。结果 miR-130a在肝癌组织中的表达显著高于对应的癌旁组织(P=0.001),miR-130a在肝癌细胞系中的表达也明显高于正常肝细胞(P=0.000)。Transwell实验结果显示,与对照组细胞相比,通过miR-130a抑制剂降低miR-130a水平的高转移性肝癌细胞系(MHCC97-H、HCCLM3)侵袭和转移的能力明显降低(P<0.01)。Western Blot显示下调miR-130a水平的MHCC97-H、HCCLM3肝癌细胞中MMP-9和MMP-2的表达水平均明显下降,Western Blot条带灰度明显降低(P<0.01)。结论 miR-130a在肝癌组织和高转移性肝癌细胞株中的高表达导致肝癌细胞的转移和侵袭增加,这一影响可能由miR-130a通过一定的信号通路促进肝癌细胞EMT而造成。Objective To find out miR-130 a expression in hepatocellular carcinoma（HCC） and its impact on migration and invasion of hepatocellular carcinoma. Methods The expression levels of miR-130 a in HCC and highly metastatic HCC cell lines（MHCC97-H, HCCLM3） were determined by qR T-PCR. Then the expression levels of miR-130 a were down-regulated by miR-130 a inhibitor in highly metastatic HCC cell lines and the impact on migration and invasion was testified by series of Transwell tests. And the expression changes of MMP-9 and MMP-2 were measured by Western Blot in order to discover the possible pathway of miR-130 a in regulating the migration and invasion of HCC. Results Expression of miR-130 a in hepatocellular carcinoma was significantly higher than the corresponding adjacent tissues（P=0.001）. The miR-130 a expression in HCC cell lines was significantly higher than in normal liver cells（P=0.000）. Transwell results showed that, compared with control cells, migration and invasion of both MHCC97-H and HCCLM3 were significantly influenced by reducing the level of miR-130 a when transfected with its inhibitor（P〈0.01）. Western Blot results found that expression of MMP-9 and MMP-2 decreased profoundly when miR-130 a were downregulated in MHCC97-H and HCCLM3 cell lines（P〈0.01）. Conclusion This study demonstrates that miR-130 a promotes the process of EMT in liver cancer cells by certain signaling pathways and molecular mechanisms, resulting in increased migration and invasion of liver cancer cells. The target genes and the corresponding signal pathways of miR-130 a are worthy of further study. miR-130 a may become one of the molecular therapy targets of liver cancer.

关 键 词：肝细胞癌 微RNA 肿瘤转移和侵袭 

分 类 号：R735.7[医药卫生—肿瘤]